CuTAPc-Fe_3O_4纳米复合粒子及其漆酶固定化研究  

Study of CuTAPc-Fe _3O_4 Nanoparticles and Their Laccase Immobilization

作　　者：黄俊[1] 周菊英[1] 肖海燕[1] 龙胜亚[1] 王军涛[1] 

机构地区：[1]武汉理工大学光纤传感技术研究中心光纤传感技术与信息处理教育部重点实验室,武汉430070

出　　处：《化学学报》2005年第14期1343-1347,i005,共6页Acta Chimica Sinica

基　　金：国家自然科学基金(No.60377032)资助项目.

摘　　要：漆酶的固定化研究对基于漆酶催化的光纤生物传感器具有十分重要的意义.制备了四氨基酞菁铜(CuTAPc)-Fe3O4纳米复合粒子,并用红外(IR)、场发射扫描电镜(FEG-SEM)、X射线衍射(XRD)、能谱、粒径仪等对其进行了表征.结果表明形成了以CuTAPc包覆在Fe3O4纳米粒子表面的纳米复合粒子,粒子呈现不规则球形,且分布均匀,粒子平均粒径在50nm左右.用此纳米复合粒子通过戊二醛交联法固定了漆酶,固定后的酶比游离酶具有更好的贮存稳定性及操作稳定性.这为研制高性能的光纤生物传感器打下了较好的基础.The immobilization of laccase is important for the fiber optic biosensor based on enzyme catalysis. Copper tetraaminophthalocyanine-ferrorerric oxide [(CuTAPc)-Fe3O4] nanoparticle composites were prepared and characterized by IR, field emission gun scanning electron microscopy, XRD, X-ray photoelectron spectroscopy and particle size analyzer. It has been proved that the CuTAPc could disperse spontaneously onto the surface of Fe3O4 nanoparticles to form molecular dispersion layer and the Fe3O4 nanoparticles were encapsulated by CuTAPc. The nanoparticle composites took the shape of roundish spheres with their mean diameter of about 50 nm. The laccase was immobilized on the surface of the composite by cross-linking with glutaraldehyde, and the immobilized laccase has better stabilities in storage and operation than free laccase. This work provided good basis for developing the fiber optic biosensor with excellent properties.

关 键 词：纳米复合粒子 酶固定化 场发射扫描电镜 光纤生物传感器 戊二醛交联法 X射线衍射 FE3O4 操作稳定性 贮存稳定性 纳米粒子 平均粒径 酶催化 酞菁铜 不规则 游离酶 漆酶 

分 类 号：O629.8[理学—有机化学]