利用特异PCR引物进行分子标记辅助选择的研究  

Study on Molecular Marker Assisted Selection with Special PCR Primers

作　　者：徐如宏[1] 任明见[1] 张庆勤[1] 

机构地区：[1]贵州大学麦作研究中心,贵阳550025

出　　处：《种子》2005年第7期15-18,共4页Seed

基　　金：贵州省"十五"攻关项目(15001A9;(2001)1112);贵州省优秀科技教育人才省长专项资金项目

摘　　要：利用与抗白粉病基因相连锁的RAPD分子标记和控制1Dy10基因序列的特异PCR引物对贵农775的杂交组合后代进行了分子标记辅助选择。在50株F2植株中,初步筛选到具有抗白粉病基因标记和5+10亚基特异标记的9株;有抗白粉病基因标记和2+12亚基特异标记的24株;有抗白粉病基因标记,同时具有5+10亚基和2+12亚基特异标记的5株。本研究说明分子标记是检测抗病基因和辅助选择育种的有效手段。The RAPD-based markers linking with powdery mildew resistance gene and the special PCR primers controlling the gene sequence of 1Dy10 were used for molecular marker assisted selection in hybrid offspring of Gui-nong wheat 775.9 Plants containing powdery mildew resistance gene marker and subunit pair 5+10 marker, 24 plants containing powdery mildew resistance gene marker and subunit pair 2+12, and 5 plants containing powdery mildew resistance gene marker and subunit pair 5+10, 2+12 were selected form 50 plants of the F_2 population. The result showed that the molecular marker assisted selection were an effective method to detect resistance gene and molecular marker assisted breeding.

关 键 词：分子标记辅助选择 PCR引物 RAPD分子标记 5+10亚基 杂交组合后代 抗白粉病基因 基因标记 辅助选择育种 基因序列 抗病基因 植株 F2 

分 类 号：S511[农业科学—作物学] S512.1