两种分离人胎盘组织单个核细胞方法的比较  

Comparison of two methods for isolating mononuclear cells from human placenta tissues

作　　者：章涛[1] 陈代雄[1] 方宁[1] 刘祖林[1] 刘金伟[1] 万卫红[1] 

机构地区：[1]遵义医学院附属医院细胞工程重点实验室,贵州遵义563003

出　　处：《遵义医学院学报》2005年第3期235-236,239,共3页Journal of Zunyi Medical University

基　　金：贵州省科技计划资助项目(黔科合计字[2003]JGY005)

摘　　要：目的探讨从人胎盘组织制备高丰度单个核细胞(MNC)的方法。方法采用机械法加胶原酶消化法制备人胎盘组织单个细胞悬液,再分别用羟乙基淀粉(6%HES)和Ficoll分离其中的MNC,并对分离效果进行比较。结果胎盘组织单个细胞悬液经6%HES分离后MNC的回收率为(45.3±11.7)%,而经Ficoll分离为(24.1±5.5)%,两者比较差异有统计学意义(P<0.01),前者分离MNC的回收率明显高于后者。结论6%HES分离法优于Ficoll法,其可从胎盘组织获得高丰度的MNC。Objective To investigate the protocols of isolating mononuclear cells (MNC) from human placenta tissues(PT).Methods Single cells suspension liquid of human PT was prepared by mechanical methods combined with collagenase digestion. Then, MNC derived from PT was separated by 6% Hydroxyethyl Starch(HES) and Ficoll respectively.Results The recovery of MNC by 6%HES and Ficoll was (45.3±11.7)% and (24.1±5.5)%,respectively.It was obviously that the MNC recovery by 6%HES was much higher than Ficoll separating methods.Conclusions In comparison to Ficoll , 6%HES separating method could acquire high recovery of MNC from human placenta tissues.

关 键 词：人胎盘 单个核细胞 分离方法 回收率 

分 类 号：R331[医药卫生—人体生理学]