白蛋白钴结合试验测定缺血修饰白蛋白  

Measurement of Ischemia Modified Albumin by Albumin Cobalt Binding Test

作　　者：苏建友[1] 鞠少卿[1] 王惠民[1] 王跃国[1] 朱健华[2] 陈莲英[1] 曹金德[1] 倪隽[2] 尚庆毅[3] 

机构地区：[1]南通大学附属医院检验医学中心,江苏226001 [2]南通大学附属医院心内科,江苏226001 [3]江苏大学

出　　处：《中国交通医学杂志》2005年第3期210-211,214,共3页Chinese Medical JOurnal of Communications

基　　金：南通市社会发展科技计划(NO.S40009)

摘　　要：目的:建立缺血修饰白蛋白(IMA)测定方法,并对正常值进行初步研究.方法:在HITACHI-7600全自动生化分析仪上设计合理参数,采用白蛋白钴结合(ACB)试验测定IMA,对其分析性能进行研究;用所建方法测定100名正常人血清IMA含量,初步制定正常人IMA的参考值.结果:所建立ACB法测定低、中、高浓度IMA的批内CV分别为2.52%、2.41%、1.96%,批间CV分别为2.56%、3.42%、3.06%;白蛋白浓度在20～50g/L范围内呈线性关系,方法的精密度和线性均佳.正常人IMA95%参考上限值为:73.0ABSU/ml.结论:在HITACHI-7600全自动生化分析仪成功建立ACB法测定MA的方法.Objective:To establish albumin cobalt binding test for measurement of ischemia modified albumin (IMA) and study its normal reference values. Methods:IMA were detected by HITACHI-7600 autobiochemical analyzer with albumin cobalt binding test and evaluated its analytical performance. Study of IMA reference values were established with 100 healthy controls. Results:The coefficient of variation values of both intra-experimental and inter-experimental reproducibility for low concentration sample were 2.52% and 2.56%, and those for medium concentration sample were 2.41% and 3.42%,and those for high concentration sample were 1.96% and 3.06% respectively. The detection range of the assay was from concentration of albumin 20.0 g/L to 50.0g/L. 95%upper limit values were 73.2ABSU/1ml. Conclusions:This assay had high sensitivity and reproducibility, and suitable for clinical practice, which will certainly play a significant role in approaching relationship between IMA and associated diseases.

关 键 词：缺血修饰白蛋白 白蛋白钴结合试验 全自动生化分析 参考值 

分 类 号：Q512.1[生物学—生物化学]