泛素-蛋白酶体抑制剂MG-132对食管癌细胞凋亡和生存素表达的影响  

Effects of Ubiquitin-proteasome Inhibitor MG-132on Apoptosis and Survivin Expression of Esophageal Carcinoma Cells

作　　者：张卫国[1] 谢国建[1] 王启斌[1] 王小虎[1] 吴清明[1] 王强[1] 童强[1] 李胜保[1] 

机构地区：[1]湖北郧阳医学院附属医院,十堰市442000

出　　处：《中国药房》2005年第14期1055-1057,共3页China Pharmacy

摘　　要：目的:研究泛素-蛋白酶体抑制剂MG-132对食管癌细胞凋亡和生存素(survivin)表达的影响。方法:将泛素-蛋白酶体抑制剂MG-132加入食管癌细胞Eca9706中,采用MTT法测定细胞生长抑制率,经流式细胞仪检测细胞凋亡,免疫细胞化学法检测survivin的表达。结果:MG-132对食管癌细胞生长具有显著抑制作用,作用24、48、72、96h后的IC50分别为120.2、18.1、—12.2、—16.9μmol/L;5.0μmol/L的MG-132作用于食管癌细胞24、48、72、96h后,细胞凋亡率分别为(3.1±0.4)%、(31.7±3.5)%、(50.4±4.8)%、(66.6±6.2)%;Survivin在食管癌细胞中呈高表达,经MG-132作用后,表达明显降低。结论:MG-132能显著抑制食管癌细胞的增殖并诱导其凋亡,其作用机制可能与下调survivin表达有关。OBJECTIVE:To investigate effects of ubiquitin-proteasome inhibitor MG-132on apoptosis and survivin expression of esophageal carcinoma cells.METHODS:The esophageal carcinoma cells Eca9706were treated with MG-132,the growth inhibitory rate was determined with MTT assay,apoptosis was detected by flow cytometry,expression of survivin was detected by immunocytochemical technique.RESULTS:MG-132had obvious inhibitory effects on the growth of gastric carˉcinoma cells,IC 50 of24hrs,48hrs,72hrs and96hrs were120.2,18.1,—12.2,and—16.9μmol/L respectively;Treated with5.0μmol/L MG-132for24hrs,48hrs,72hrs and96hrs,apoptotic rates of cells were(3.1±0.4)%、(31.7±3.5)%、(50.4±4.8)%and(66.6±6.2)%respectively;Expression of survivin was high in esophageal carcinoma cells and it was decreased in cells treated with MG-132.CONCLUSIONS:MG-132can significantly inhibit the proliferation of esophageal carcinoma cells and induce apoptosis,which might be associated with down-regulated expression of survivin.

关 键 词：泛素-蛋白酶体抑制剂 食管癌 凋亡 SURVIVIN 

分 类 号：R965[医药卫生—药理学] R979.1[医药卫生—药学]