共振光散射探针法测定壁画胶结材料中蛋白质含量  

Determination of Proteins in the Binding Material of Wall Painting Pigments with Acid Chrome Blue K as a Probe of Resonance Light Scattering

作　　者：陈广德[1] 朵吉华[1] 

机构地区：[1]西北师范大学化学化工学院,甘肃兰州730070

出　　处：《分析测试学报》2005年第4期6-9,共4页Journal of Instrumental Analysis

基　　金：国家文物局文物保护基金资助项目(9908)

摘　　要：用酸性铬蓝K(ACBK)为共振光散射探针,对壁画含不同颜料的胶结材料中的蛋白质含量进行了定量测定.在pH 3.96乙酸-乙酸钠缓冲溶液条件下,在λ=345 nm处,以牛血清白蛋白(BSA)为标准样品绘制工作曲线.测定敦煌壁画中含不同颜料的胶结材料中蛋白质结果分别为:白色颜料1.536 1 μg/mg;绿色颜料1.571 4g/mg;蓝色颜料1.680 1μg/mg;棕色颜料1.875 6 μg/mg和红色颜料3.267 3 μg/mg.对BSA测定的线性范围为0.13～10.88 mg/L.该方法简单、快速、灵敏度高,平行测定的相对标准偏差(骼D)为3.8%,蛋白质的加标回收率为95%～107%.Acid Chrome Blue K(ACBK)was used as a probe of resonance light scattering(RLS)to determine the proteins in the binding material of wall painting pigments.The RLS was generated through the interaction between ACBK and proteins in sodium acetate-acetic acid buffer solution at pH3.96.Bovine serum albu-min(BSA)was used as the standard sample for calibration.A linear relationship between the RLS intensity atλ=345nm and the concentration of BSA was observed in the range of0.136-10.88mg/L.The method was applied to the determination of proteins in the binding material of wall painting pigments at Dunhuang.The results for1mg of white,green,blue,brown and red pigments were1.5361,1.5714,1.6801,1.8756and3.2673μg/mg,respectively.The relative standard deviation was3.8%and the recoveries of proteins were in the range of95%-107%.The method is simple,rapid and sensitive.

关 键 词：共振光散射 蛋白质 酸性铬蓝K 壁画胶结材料 

分 类 号：O657.39[理学—分析化学] Q51[理学—化学]