Ru(phen)_3^(2+)-SDBS-KMnO_4化学发光体系检测还原型谷胱甘肽的研究  

Chemiluminescence Determination of Reduced Form Glutathione with Ru(phen)_3^(2+)-SDBS-KMnO_4 System

作　　者：张韶虹[1] 郗娟[1] 吉邢虎[1] 何治柯[1] 

机构地区：[1]武汉大学化学与分子科学学院

出　　处：《分析测试学报》2005年第4期48-50,53,共4页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(20275028)

摘　　要：当硫酸介质中有阴离子表面活性剂十二烷基苯磺酸钠(SDBS)存在时,还原型谷胱甘肽(G-SH)可以增强三(1,10-菲咯啉)钌(Ⅱ)[Ru(phen)23+]-KMnO4化学发光体系的发光强度。基于此,建立了一种化学发光直接检测G-SH的新方法。在优化的实验条件下,该方法的线性范围和检出限分别为5.0×10-2~2.0μg·mL-1和3.5×10-2μg·mL-1,对1.0μg·mL-1的G-SH溶液平行测定11次的相对标准偏差(RSD)为5.8%。将其用于合成样品中G-SH含量的测定,结果满意。并提出了可能的化学发光机理。A novel chemiluminescence(CL)method for the determination of reduced form glutathione(G- SH)was presented.It was based on the fact that the CL intensity of tris-(1,10-phenanthroline)ruthenium(Ⅱ)( 2+ Ru(phen) 3 )-KMnO 4 system could be greatly enhanced by the reduced form G-SH in the presence of sodium dodecyl benzene sulfonate(SDBS).Under optimum experimental conditions,the CL intensity was proportional to the G-SH concentration in the range of5.0×10 -2 -2.0μg·mL -1 and the detection limit was3.5×10 -2 μg·mL -1 .The relative standard deviation(n=11)of1.0μg·mL -1 G-SH in solution was5.8%.This method has been successfully applied for the determination of G-SH in synthetic samples.The mechanism of CL reaction was also studied.

关 键 词：还原型谷胱甘肽 化学发光 三(1.10-菲咯啉)钌(Ⅱ) 

分 类 号：O657.3[理学—分析化学]