葛根素对胰岛素抵抗大鼠骨骼肌中GSK-3表达的影响  

Effects of Puerarin on the Expression of Glycogen Synthase Kinase- 3 in Skeletal Muscle of Rats with Insulin Resistance

作　　者：陈丹[1] 毕会民[1] 苏杭[1] 

机构地区：[1]武汉大学人民医院内分泌科,武汉430060

出　　处：《中药新药与临床药理》2005年第4期233-236,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：湖北省自然科学基金资助课题(编号2003ABA182)。

摘　　要：目的观察葛根素对高脂高糖饮食诱导的胰岛素抵抗模型大鼠骨骼肌中糖原合成酶激酶-3(GSK-3)表达的影响。方法将30只Wistar大鼠随机分为正常组、模型组和葛根素组,每组10只。模型组和葛根素组均喂以高糖高脂饲料,4周后葛根素组大鼠给予100mg/kg葛根素腹腔注射,连续6周。定期检测其体重、血甘油三酯和胆固醇、空腹血糖和胰岛素水平,并计算胰岛素敏感指数。实验结束后采用Westernblot法检测各组大鼠骨骼肌中GSK-3的含量。结果模型组大鼠骨骼肌中GSK-3的含量较正常组增高70.20%(P<0.01),给予葛根素治疗6周后,大鼠骨骼肌中GSK-3的含量较模型组减少26.54%(P<0.01),较正常组增高25.02%(P<0.05)。结论葛根素可降低胰岛素抵抗大鼠骨骼肌细胞GSK-3蛋白表达水平,从而加强葡萄糖的摄取和利用,改善胰岛素抵抗。Objective To observe the effects of puerarin on the expression of glycogen synthase kinase- 3 (GSK- 3) in the skeletal muscle of rats with insulin resistance induced by high fat and sucrose diets. Methods 30 Wistar rats were randomly divided into three groups: normal group, model group and puerarin group, 10 rats in each group. The model group and puerarin group were fed with the high fat and sucrose diets for 4 weeks. Then puerarin group was treated with puerarin by abdominal injection (100 mg/kg) for 6 weeks. At the end of the experiment, GSK- 3 content was detected by Western blot analysis. Body weight, serum triglyceride and cholesterol levels, fasting plasma glucose and serum insulin concentrations were measured regularly and insulin sensitivity index was also computed as well. Results The outcome of Western blot analysis showed that the expression of GSK- 3 in the skeletal muscle of the model group increased by 70.20 % (P < 0.01) compared with the normal group. After 6 weeks of puerarin treatment, the GSK- 3 content in the skeletal muscle of the puerarin group decreased by 26.54 % (P < 0.01) compared with the model group and increased by 25.02 % (P < 0.05) compared with the normal group. Conclusion Puerarin can lower the expression of GSK- 3 in the skeletal muscle of rats with insulin resistance, consequently promoting the ingestion and utilization of glucose and improving the insulin resistance.

关 键 词：葛根素 糖原合成酶激酶-3 胰岛素抵抗 动物模型 

分 类 号：R285.5[医药卫生—中药学]