舌苔变化与凋亡相关基因分子机理的研究  

STUDY ON RELATIONSHIP BETWEEN CHANGES OF TONGUE FUR AND CORRELATION GENE EXPRESSION OF APOPTOSIS

作　　者：盛光[1] 吴正治 李明 张永锋 陈嫚茵 

机构地区：[1]深圳市第二人民医院,深圳518029 [2]深圳市中西医结合研究所,深圳518029

出　　处：《中国中医药科技》2005年第4期201-202,i001,共3页Chinese Journal of Traditional Medical Science and Technology

基　　金：国家自然科学基金资助项目No.39770890

摘　　要：目的:检测常见舌苔变化与舌上皮细胞凋亡情况及凋亡相关基因TGF-β3与fasmRNA和蛋白产物,探讨舌苔变化与舌上皮细胞凋亡、凋亡相关基因表达的关系。方法:运用TUNEL技术、原位杂交、免疫组化和图像分析技术。结果:与正常薄苔比较,剥苔fas基因过度表达伴随细胞凋亡增多,而厚苔TGF-β3基因低表达伴随细胞凋亡减少。舌苔上皮细胞中促凋基因fas、TGF-β3表达水平变化趋势与细胞凋亡水平变化趋势一致。结论:凋亡相关基因fas、TGF-β3表达水平的变化可能是影响舌苔上皮细胞凋亡并导致舌苔厚度变化的重要原因。Objective: To investigate the relationship between the gene expression of bax, fas, TGF -β3 and cell apoptosis in tongue mycoderma epithelium of common tongue coating. Methods: TUNEL(terminal deoxynucleotidyl transferase - mediated d - UTP nick - end labeling)technique,in stitu hybridization, immunohistochemical technique and image analysis were used in the study. Results: In comparison with normal tongue fur, there was an over - expression of bax and fas in peeling fur, accompaning with, up - regulation of apoptosis, while there was an lower - expression of bax and TGF - β3 in thick fur accompaning with down - regulation of apoptosis. The changes of bax, fas, TGF - β3 gene expression level had the same tendency with that of cell apoptosis level. Conclusion: The results suggested that the change of expression level of bax, fas, TGF - β3 effect cell apoptosis in tongue mycoderma epithelium maybe an important cause for the thickness' s change of tongue fur.

关 键 词：舌苔 细胞凋亡 基因表达 舌诊客观化 人类 

分 类 号：R241[医药卫生—中医诊断学]