天麻素对Aβ25-35诱导的Alzheimer病细胞模型的保护作用  

Protective Effects of Gastrodin on the Cellular Model of Alzheimer's Disease Induced by Aβ25-35

作　　者：刘中华[1] 胡海涛[1] 冯改丰[1] 赵志英[1] 毛乃颖[2] 

机构地区：[1]西安交通大学医学院人体解剖学教研室,西安710061 [2]中国疾病预防控制中心病毒预防控制所

出　　处：《四川大学学报（医学版）》2005年第4期537-540,共4页Journal of Sichuan University(Medical Sciences)

摘　　要：目的研究天麻素(GAS)对诱导的阿尔茨海默病(AD)神经细胞的作用。方法以β-淀粉样肽25-35片段(Aβ25-35)20μmol/L诱导原代培养的大鼠大脑皮层、海马神经细胞建立AD细胞模型,以人参总皂甙作为阳性对照,通过形态学观察、MTT等方法检测细胞活力。结果与对照组比较,Aβ25-35诱导的培养神经元发生明显的形态学变化,MTT检测显示细胞活力明显下降,乳酸脱氢酶(LDH)释放增多,与对照组比较,差异有显著性(P<0.05);当预先加入不同质量浓度的天麻素、人参总皂甙时,Aβ25-35对神经细胞的损伤明显减轻,细胞活力明显增强,LDH释放减少,与实验对照组对比差异均有显著性(P<0.05)。结论天麻素具有预防和治疗AD的潜在功效。Objective To assess the effects of gastrodin(GAS) on the induced neurons of Alzheimer's disease(AD). Methods The cellular model of AD was established with primary cultured cerebral cortical and hippocampal cells induced by Aβ25-35. The total saponin ginseng was taken as positive control. The effects of gastrodin on the cellular model of AD were studied by morphological observation and biochemical methods. Results (Compared with) the control, the cellular model induced by Aβ25-35 showed remarkable morphological changes, significant increase in LDH release, and significant decrease in cell survival. When different doses of gastrodin, total saponin of ginseng were added beforehand, significant decrease in LDH release and significant increase in cell survival were noted by comparison with the experimental control, which indicated that the two medicines could protect the cells from the damage induced by Aβ25-35 to some extent. Conclusion The results suggested that gastrodin has potential effects on the prevention and therapy of AD.

关 键 词：阿尔茨海默病 天麻素 Β-淀粉样肽 细胞培养 

分 类 号：R285.5[医药卫生—中药学]