我国主要地方绵羊品种mtDNA细胞色素b基因PCR-RFLP研究  

Study on mtDNA Cyt b Gene of Chinese Main Indigenous Sheep Breeds Using PCR-RFLP

作　　者：巩元芳[1] 李祥龙[2] 刘铮铸[1] 

机构地区：[1]河北科技师范学院动物科学系,昌黎066600 [2]河北农业大学动物科技学院,保定071001

出　　处：《畜牧兽医学报》2005年第7期649-653,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：河北省自然科学基金(302423)

摘　　要：应用PCRRFLP方法,对我国9个地方绵羊品种和3个引入品种共计120个个体的线粒体DNA(mtDNA)细胞色素b(cytb)基因进行了分析。结果表明,12种限制性内切酶在所研究的个体中共检测到22个酶切位点,15种限制性态型,其中StuⅠ、EcoRⅠ和MboⅡ表现出多态,15种限制性态型归结为4种基因单倍型,即单倍型Ⅰ、单倍型Ⅱ、单倍型Ⅲ和单倍型Ⅳ。单倍型Ⅰ为受试绵羊品种的基本单倍型。线粒体DNA细胞色素b基因遗传多态度为0.0142%,说明我国地方绵羊品种线粒体DNA多态度贫乏。Technique of polymerase chain reaction(PCR) and method of restriction fragment length polymorphism(RFLP) were performed to analyse Cytochrome b(Cyt b) gene of mtDNA in 9 indigenous sheep breeds and 3 imported sheep breeds.The results indicated that 22 restriction sites and 15 restriction morphs were detected with the digestion of 6 endonucleases in all individuals,and StuⅠ, EcoRⅠ and MboⅡ were polymorphic.Fifteen restriction morphs were sorted into 4 haplotypes.The haplotype I was the main haplotype.The averaged polymorphic degree(π value=0.014 2%) of mtDNA Cyt b gene showed that the genetic diversity of mtDNA of Chinese indigenous sheep breeds was very poor.

关 键 词：地方绵羊品种 线粒体DNA 细胞色素B基因 PCR-RFLP 

分 类 号：S826.2[农业科学—畜牧学] Q953[农业科学—畜牧兽医]