自制KYL液和UW液保存大鼠肝脏效果的比较  

Comparision of Self-Designed KYL Solution with UW Solution for Rat Liver Preservation

作　　者：李立[1] 李春满[1] 李晓延[1] 胡明道[1] 冉江华[1] 黄明[1] 张炳彦[1] 

机构地区：[1]昆明医学院第二附属医院肝胆胰一病区,昆明650101

出　　处：《中国普外基础与临床杂志》2005年第4期375-379,383,共6页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的比较自制的KYL液和UW液对大鼠肝脏的保存效果。方法采用大鼠肝脏非循环离体灌注模型(noncirculatedisolatedperfusionofratliver),随机以KYL液和UW液对大鼠肝脏保存0、4、8、16、24和48h,记录胆汁流出量,测定灌注流出液天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、乳酸脱氢酶(LDH)和氧自由基代谢产物丙二醛(MDA)及超氧化物歧化酶(SOD)含量,检测肝细胞内钙离子浓度,观察肝脏组织形态学变化。同时设生理盐水保存阴性对照组,了解器官保存液对大鼠肝脏有无保护作用。结果KYL液保存的大鼠肝脏在保存16h以内各时相胆汁流出量均较UW液保存者高(P<0.01),灌注流出液AST、ALT和LDH含量与UW液保存者相近,肝细胞内钙离子浓度较UW液保存者低(P<0.01);KYL组保存24及48h时,MDA含量低于UW组,SOD含量高于UW组(P<0.01);光、电镜观察两者形态学变化基本一致。两组所有指标均较生理盐水保存组好,提示KYL保存与UW液一样对大鼠肝脏具有保护作用。结论自制的KYL液对大鼠肝脏的保存效果总体上与UW液相当,在再灌注后肝细胞胆汁分泌方面和钙拮抗方面略优于UW液,而在防止细胞水肿方面较UW液稍差。Objective To compare the effects of self-designed KYL solution on rat liver preservation with UW solution. Methods Using non-circulated isolated perfused rat liver,SD rat livers were randomly preserved for 0、4、8、16、24 and 48 hours with self-designed KYL solution or UW solution. The effects were assessed by measuring the bile production, the contents of AST, ALT,LDH,free radical( MDA) and SOD in Krebs-Henseleit’s perfusate and the content of intracellar calaium of the rat hepatocytes, and by observing the morphological changes in liver. At the same time, the normal saline was used to preserve the rats liver as negative comparison to know if KYL solution and UW solution can prevent the ischemia-reperfusion injury in rat liver transplantation.Results At different periods within 16 hours of preservation,the bile production of the rat livers which were preserved by KYL solution were more than those were preserved by UW solution (P<0.01), The contents of AST, ALT and LDH in Krebs-Henseleit’s perfusate were near in UW solution’s. The content of intracellar calaium of the rat hepatocytes which were preserved by KYL solution were less than those were preserved by UW solution (P<0.01). In KYL group, the content of MDA was lower than that UW group, the content of SOD was higher than that UW group at 24 and 48 h((P<0.01)). Morphological changes in both groups were almost same. All variables of both groups were better than the rat livers preserved by the normal saline. It was proved that both KYL solution and UW solution could protect the rat livers. Conclusion The study indicated that the effects of self-designed KYL solution on rat liver preservation are similar to that of UW solution. The bile production after reperfusion and the content of intracellar calaium of the rat hepatocytes in KYL group are better than those in UW group .The effect of prevention of rat hepatocellular oedema by self-designed KYL solution are worse than that of UW solution.

关 键 词：肝脏保存 大鼠 UW液 KYL液 

分 类 号：R657.3[医药卫生—外科学] R-332[医药卫生—临床医学]