^(99)Tc^m-MIBI在癌组织中的摄取与跨膜电位或肺耐药蛋白相关性  

Correlations between ^(99)Tc^m-MIBI uptake and membrane potential of mitochondrion or lung resistant protein of tumor tissues

作　　者：胡硕[1] 付春燕[2] 黄柏英[3] 梁昌华[1] 

机构地区：[1]中南大学湘雅医院核医学科,湖南长沙410008 [2]中南大学湘雅医院病理科,湖南长沙410008 [3]中南大学湘雅医院现代测试中心,湖南长沙410008

出　　处：《中国医学影像技术》2005年第7期1078-1081,共4页Chinese Journal of Medical Imaging Technology

基　　金：湖南省科技厅课题基金(03SSY3054)资助。

摘　　要：目的探讨99TcmMIBI检测肺腺癌A549DDP细胞的耐药性的机制。方法用MTT法检测顺铂对敏感A549细胞的IC50及有或无顺铂再处理后的A549DDP细胞的IC50。将上述细胞注入裸鼠皮下建立模型后,用99TcmMIBI进行SPECT显像,以免疫组化方法检测移植瘤细胞的LRP蛋白表达,流式细胞仪检测癌细胞的跨膜电位。结果顺铂对A549细胞、A549DDP细胞的IC50分别为24、325、182μM。A549裸鼠99TcmMIBI显像可见瘤块放射性浓聚影,A549DDP裸鼠亦可见瘤块的放射性浓聚影,其放射性浓度较前者明显减淡(P<0.05)。A549移植瘤细胞LRP蛋白呈阴性,无顺铂再处理的A549DDP移植瘤细胞LRP蛋白表达呈阳性,顺铂再处理的A549DDP移植瘤细胞LRP蛋白表达呈强阳性,三者间有显著性差异(P<0.01)。各组细胞的LRP蛋白的表达与99TcmMIBI摄取值呈弱的负相关(r=-0.59,P<0.05)。三种细胞跨膜电位有显著性差异,跨膜电位与99TcmMIBI摄取值呈较强的负相关(r=-0.71,P=0.005)。结论99TcmMIBI检测肺腺癌耐药的A549DDP细胞的耐药性与细胞的跨膜电位关系密切,能在某种程度上反映肺癌LRP蛋白的表达。Objective To study the mechanism of 99 Tcm -MIBI examining the resistance of lung adenocarcinoma A549DDP cells. Methods The method of methoxyisobutylisonitrile (MTT) was applied to test IC_ 50 of cisplatin to A549 cells, to test IC_ 50 of cisplatin to A549DDP cells after dealing with or without micro dose of cisplatin. The cells above were all injected under the skin of nude mice to observe growth status of tumors. After setting up of the tumor models, the nude mice were performed using SPECT with 99 Tcm -MIBI. Lung resistant protein (LRP) of transplanted tumor cells were determined with immunohistochemical method. Membrane potential of mitochondrion (△Ψ) of the cells were measured by flow cytometry. Results IC_ 50 of cisplatin to A549 cells was 24 μM. That to A549DDP cells which did not deal with micro dose of cisplatin was 182 μM, and to A549DDP cells dealed with mecro dose of cisplatin was 325 μM. Tumors developed by reason of incepting 99 Tcm -MIBI in all mice. The R value in A549 mice was remarkably higher than that in A549DDP mice (P<0.05). LRP in A549 cells from transplanted tumor did not express otherwise that in A549DDP cells strongly expressed. △Ψ of A549DDP cells dealt with micro dose of cisplatin were observably higher than that of both A549DDP cells which dealt without micro dose of cisplatin and A549 cells. Conclusion The mechamism of 99 Tcm -MIBI examine the resistance of lung adenocarcinoma A549DDP cells may correlate with both △Ψ of cells and expression of LRP of cells.

关 键 词：^99TC^M-MIBI 耐药 跨膜电位 肺耐药蛋白 

分 类 号：Q95-33[生物学—动物学]