血小板浓缩液的制备及促进兔尺骨骨折愈合方面的实验研究  

Experimental study on the preparation of blood platelet concentrate and its promotion to fracture healing of rabbit ulna

作　　者：张爱民[1] 张英泽[2] 马维[1] 范志勇[1] 史正亮[1] 宋永周[1] 张华[1] 郑广德[1] 

机构地区：[1]河北医科大学第二附属医院骨科,河北石家庄050061 [2]河北医科大学第三附属医院骨科

出　　处：《中国骨伤》2005年第6期346-348,共3页China Journal of Orthopaedics and Traumatology

摘　　要：目的:探索动脉血小板浓缩液,在无菌情况下的制备方法。通过免疫组化及物理测量,证实血小板浓缩液中含有多种生长因子,并对比分析其对骨折愈合不同时期的促进作用,为临床应用提供基础资料。方法:选用20只新西兰兔,随机分成2组,分别为空白组及血小板组。制作尺骨中段骨折动物模型,采用指钢板行内固定。在手术之前,先抽取兔股动脉血6ml左右,经枸橼酸钠抗凝后,加低速高速离心,提纯出白色的血小板浓缩液,将其注射回骨折断端。所有操作均为无菌操作。分别在1、2、4及6周处死兔,在骨折处取5mm骨组织标本。切片作PDGF免疫组化染色定性分析及测量骨痂直径定量分析。结果:①6ml的兔动脉血在低速及高速离心后,可分离出0.5ml的血小板浓缩液,浓度在正常兔血小板浓度的3倍以上。②免疫组化染色阳性产物呈棕黄色颗粒状。在空白组术后第1周及第2周PDGF因子为阴性表达,第4周、第6周为弱阳性表达;在血小板浓缩液组,随着处死时间延长,阳性表达率逐渐增高。在相同的处死时间,血小板浓缩液组阳性表达率明显高于空白组。空白组、血小板组兔尺骨骨折标本在1、2、4、6周,均有不同程度骨痂形成,其中以第6周兔骨痂最多,第1周兔以纤维连接为主,仅有少许骨痂形成。空白组与血小板组同期骨痂直径差异有显著性意义。结论:①少量的兔血在严格的实验条件下,能够制备出血小板浓缩液。②兔血小板浓缩液中确含有较高浓度的PDGF生长因子。③血小板浓缩液加速了骨折断端的愈合。Objective:To explore the preparative method for blood platelet concentrate under the aseptic condition.Furthermore,with immunohistochemical and physical measurement,to confirm promoting effection of blood platelet concentrate which have many types of growth factors on the fracture healing of rabbit's ulna at different periods and to offer theoretical bases for clinical application. Methods:Twenty New Zealand rabbits were applied and divided into 2 groups randomly.The fracture models were made on the middle of ulna fixed by finger armor plate.Before the operation,we drew out 6 ml blood from femoral artery and centrifugated by low and the followed high speed with the anti-coagulation of sodium citrate,then purified the white blood plate and injected it into the fracture position.All the manipulation was under the aseptic condition.The rabbits were killed at 1,2,4 and 6 weeks.Then bone specimen of 5 mm was cut in length of bone tissue near the fracture.Qualitative analysis by immun-histochemical staining of PDGF and quantitive analysis by measuring bony were diametered with micrometer.Results:①Blood platelet concentrate of 0.5 ml can be acquired from rabbit's artery blood of 6 ml centrifugated by low and high speed.The concentration had three times as normal level.②Immunohisto-biochemical staining showed positive reaction as brown yellows graunle.In first and second weeks after operation,PDGF factor showed negative.In 4th and 6th weeks,weak positive.In the blood platelet concentration group,so long with the longer time,the rate of reaction becomes higher.At the same time,the positive reaction masccline rate of blood platelet concentration group was obviously higher than that of the control group.③Bony scab could be seen on the ulnar specimen in various degrees as the rabbits were killed at 1st,2nd,4th and 6th weeks,particularly in the last week.However,in first week only a small amount of callus which was conjuncted with fibers could be seen,and three were no difference from the normal bone diameter.At the sam

关 键 词：血小板浓缩液 制备方法 兔 动物模型 尺骨骨折 骨折愈合 

分 类 号：R683.41[医药卫生—骨科学]