11,12-EET对长时间保存幼兔供心再灌注后心肌细胞凋亡及bcl-2 mRNA基因表达的影响  

Effect of 11,12-EET on cardiomyocyte apoptosis and bcl-2 mRNA gene expression in donor hearts of immature rabbit underwent prolonged protection

作　　者：钟星[1] 吴清玉[2] 周明阳[2] 温复兴[3] 覃筱燕[4] 丁志敏 

机构地区：[1]中国医学科学院肿瘤研究所细胞及分子生物学实验室,北京100037 [2]中国医学科学院阜外心血管病研究所器官移植研究室,北京100037 [3]中国医学科学院麻醉与体外循环研究室,北京100037 [4]中央民族大学生物化学系,北京100089 [5]广西省人民医院病理科,广西南宁530021

出　　处：《中国病理生理杂志》2005年第7期1270-1273,共4页Chinese Journal of Pathophysiology

摘　　要：目的:观察11,12-epoxyeicosatrienoicacid(11,12-EET)作为心脏停搏液及保存液成分对未成熟兔离体心脏长时间保存后经再灌注,心肌细胞凋亡及bcl-2mRNA基因表达的变化,并探讨其可能机制及意义。方法:利用非循环式Langendorff灌注装置将24只未成熟离体兔心制成实验模型,按单纯随机分配原则分成正常对照组(心脏未经停搏及再灌注处理)、托马斯(ST)液对照组(St.ThomasNo.2液为停搏液及保存液处理)和EET组(St.ThomasNo.2液+11,12-EET为停搏液及保存液处理)。ST对照组及EET组心脏保存24h(4℃)后再灌注30min。采用TUNEL法观察各组心肌细胞凋亡数以及原位杂交方法检测心肌bcl-2mRNA基因表达等指标。结果:(1)ST对照组及EET组的心肌细胞凋亡数均高于正常对照组。(2)EET组的心肌细胞凋亡数明显低于ST对照组,bcl-2mRNA基因表达则显著高于ST对照组。结论:本研究采用不同方法在供体心脏保存过程中,均出现不同程度的心肌细胞凋亡。然而,11,12-EET能够明显减少心肌细胞凋亡。上调凋亡抑制基因bcl-2mRNA表达可能是11,12-EET减少心肌细胞凋亡的机制之一。AIM: To study apoptosis and bcl-2 mRNA gene expression of cardiomyocytes in donor hearts of immature rabbits underwent prolonged protection by 11, 12-epoxyeicosatrienoic acid (11, 12-EET), and further probe into the possible mechanisms. METHODS: 24 isolated immature rabbit hearts were performed to the model in a Langendorff perfusion apparatus and randomly assigned to normal control group,ST control group and EET group. The isolated rabbit hearts in ST control group and EET group were stored for 24 hours with 4 ℃ hypothermia, and underwent 30 minutes of reperfusion (37 ℃). TUNEL and in situ hybridization (ISH) methods were applied in the present study and apoptotic cells and bcl-2 mRNA gene expression were observed. RESULTS: The numbers of apoptotic cardiomyocytes in ST group and EET group were higher than that in normal control group, and the numbers of apoptotic cardiomyocytes were significantly decreased in EET group and bcl-2 mRNA positive expression were higher than that in ST control group, respectively. CONCLUSIONS: There were apoptosis during the prolonged protection of donor heart in our study, and we proved that: ①11,12-EET could decrease cardiomyocyte apoptosis significantly. ②Up-regulation of the bcl-2 mRNA expression in cardiomyocytes may be one of the mechanism responsible for inhibition of cardiomyocyte apoptosis by 11, 12-EET.

关 键 词：心脏 细胞凋亡 基因表达 11 12-环氧二十碳二烯酸 

分 类 号：R541.4[医药卫生—心血管疾病]