必需脂肪酸对新生大鼠小脑细胞构筑结构的影响  

Effects of essential fatty acids on the cell construction of cerebella in neonatal rats

作　　者：张侃[1] 曾琳[2] 龙在云[2] 李应玉[2] 刘媛[2] 陶翠兰[2] 伍亚民[2] 何凤慈[2] 

机构地区：[1]重庆医科大学附属儿童医院儿保科,重庆400014 [2]第三军医大学大坪医院野战外科研究所第三研究室,重庆400042

出　　处：《第三军医大学学报》2005年第13期1355-1356,共2页Journal of Third Military Medical University

摘　　要：目的探讨发育期补充必需脂肪酸(essentialfattyacid,EFA)对大鼠小脑细胞构筑发育的影响。方法新生1个月大鼠90只分为正常组、EFA缺乏组和补充鱼油组,分别以不同饲料喂养3个月,HE染色、尼氏染色和嗜银染色观察小脑细胞构筑形态结构的变化。结果EFA缺乏组与正常组相比,小脑皮层的浦肯野氏细胞数目明显减少、排列不规则、稀疏,可见大量的蓝状细胞肿胀、变性坏死,攀缘纤维、苔藓纤维数目明显减少、变短、排列稀疏不规整。EFA补充组小脑细胞构筑结构与正常无差异。结论发育期EFA缺乏可导致小脑细胞受损、细胞构筑发育异常,发育期补充EFA能促进小脑的正常发育和功能。Objective To investigate the effects of essential fatty acids (EFA) on the cell construction of cerebella in neonatal rats. Methods Ninety neonatal rats, one month old, were randomly divided into normal, EFA deficiency and fish oil supplement groups and given different feeding stuff for three months respectively. The changes of morphology and structure in cerebella were observed by hematoxylin and eosin (HE) staining,Nissl and silver staining. Results As compared with the normal group, the number of Purkinye's cells in cerebellum was much less than the fish oil supplement group and the array of cells mentioned above was atactic, and there were many basket cells which were swollen, degenerated and dead in the molecular stratum, and meanwhile the climbing fibers and mossy fibers became sparse, shorter and turbulent in EFA deficiency group. But there were remarkable differences in the cell construction of cerebellum between the normal group and the fish oil supplement group. Conclusion Deficiency of EFA at developmental period can cause the damage of neuron and cell construction in cerebellum. It is necessary for the promoting development and function of cerebellum supplemented EFA during the developing period.

关 键 词：必需脂肪酸 小脑 发育 细胞构筑 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学] R329.24[医药卫生—基础医学]