大鼠肝再生过程中增殖细胞核抗原的表达  

Expression of proliferating cell nuclear antigen during liver regeneration process after partial-hepatectomy in rats

作　　者：刘铜[1] 王冬梅[2] 

机构地区：[1]蚌埠医学院组织胚胎学教研室,安徽蚌埠233003 [2]安徽省宿州市卫生学校,234000

出　　处：《蚌埠医学院学报》2005年第4期293-294,297,共3页Journal of Bengbu Medical College

摘　　要：目的:探讨大鼠肝再生过程中肝细胞增殖细胞核抗原(PCNA)表达的变化。方法:建立肝切除动物模型,用免疫组织化学法、图像分析仪检测肝细胞PCNA的表达。结果:PCNA强阳性肝细胞数及其总灰度值在肝大部切除后24h明显增高,肝细胞阳性表达率在48h达到峰值,至120h趋于正常水平。结论:PCNA强阳性肝细胞呈规律性变化,提示残余肝可再生。Objective:To observe the expression of proliferating cell nuclear antigen(PCNA) during liver regeneration process after partial-hepatectomy in rats.Methods:ABC immunohistochemistry methods and anti-PCNA monoclonal antibody were used to reveal the expression of PCNA in the rat liver slice.Results:The number of PCNA positive cells and the grey degree numerical value increased remarkably 24 h after hepatectomy,and the expression rate of heptocytes increased to peak valuer 48 h after hepatectomy. Conclusions:The PCNA strong positive cells show regular change afterhepatectomy,which suggests that the remained liver cells can regenerate.

关 键 词：肝再生 免疫组织化学 增殖细胞核抗原 大鼠 

分 类 号：R333.4[医药卫生—人体生理学]