^(60)Coγ射线照射对AT和GM细胞增殖能力及O_2^(.-)浓度的影响  

EFFECTS OF ^(60)Co γ RAY IRRADIATION ON THE PROLIFERATION OF AT AND GM FIBROBLASTS AND O_2(.-) CONCENTRATION

作　　者：王明明[1] 朱巍[1] 曹建平[1] 罗加林[1] 郑斯英[1] 江家贵[1] 周献峰[1] 朱财英[1] 

机构地区：[1]苏州大学放射医学与公共卫生学院,215007

出　　处：《辐射防护》2005年第4期231-234,共4页Radiation Protection

基　　金：国家自然科学基金(30170288)资助项目。

摘　　要：为探求AT5BIVA细胞辐射敏感性与超氧阴离子自由基O2.-之间的联系,以正常人皮肤成纤维细胞GM0639为对照,用3HTdR掺入法和细胞色素C还原法分别测定正常及照射条件下细胞增殖能力及其培养介质中O2.-浓度(nmol/106细胞)。结果表明,在正常培养条件下,两种细胞的增殖能力和向培养介质中释放O2.-的量均随培养时间的延长而增加。在不同剂量60Coγ射线照射条件下,两种细胞的增殖能力和向培养介质中释放O2.—的量均随剂量增大而减小;两种细胞的增殖能力均与其培养介质中O2.—浓度密切相关,且AT5BIVA细胞的增殖速率大于GM0639细胞。提示AT5BIVA细胞高辐射敏感性与O2.—产生量密切相关。To study the correlation between the radiosensitivity of AT5BIVA fibroblast and superoxide anion free radical O_2 .- ,and after exposure to 60 Co γ ray,comparison was made with GM0639 fibroblast 3 H-TdR incorporation method and cytochrome C reduction method were used to determine cell proliferation(CPM)and the concentration(nmol/h/10 6 cells) of O_2 .- in culture medium under normal culture condition.The results showed that both the proliferation rate of two kinds of fibroblasts and the release of O_2 .- into the medium increase with extended time under normal culture condition,but decrease with extended time under different 60 Co γ ray exposure conditions.The proliferation rate of two kinds of fibroblasts are closely related to the concentration of O_2 .- in the medium.AT5BIVA fibroblasts proliferates at a higher rate than GM0639 fibroblasts do.The findings suggest that the high radiosensitivity of AT5BIVA fibroblast closely correlates with its O_2 .- production.

关 键 词：^60COΓ射线照射 细胞增殖能力 O2 浓度 超氧阴离子自由基 皮肤成纤维细胞 辐射敏感性 培养介质 GM AT 细胞色素C 培养条件 培养时间 增殖速率 正常人 还原法 产生量 剂量 相关 

分 类 号：R817[医药卫生—影像医学与核医学]