邻苯二甲酸二丁酯的拟雌激素活性研究  

Experimental Study on the Estrogenic Activity of Dibutyl Phthalate

作　　者：靳秋梅[1] 孙增荣[1] 

机构地区：[1]天津医科大学公共卫生学院,天津300070

出　　处：《环境与健康杂志》2005年第4期261-263,共3页Journal of Environment and Health

摘　　要：目的探讨邻苯二甲酸二丁酯(DBP)的拟雌激素活性。方法对雌激素敏感的人乳腺癌MCF-7细胞在RPMI1640培养基中采用开放式单层贴壁培养,开始实验前将细胞用PBS洗涤后改为在无酚红RPMI1640培养基中培养5d,实验设溶剂对照、雌激素阳性对照和邻苯二甲酸二丁酯各剂量组(10-7～10-3mol/L),采用四唑盐(MTT)法、生长曲线、有丝分裂指数和细胞克隆形成试验对MCF-7细胞增殖情况进行分析。结果与溶剂对照组相比,10-5mol/LDBP处理24h就可促进MCF-7细胞增殖,提高增殖指数;随着培养时间延长至96h,其他浓度DBP也表现出促进细胞增殖的效果,且浓度在10-5mol/L时,细胞增殖活性达到最大;在对数生长期,DBP可提高MCF-7细胞的有丝分裂指数;10-5mol/LDBP处理48h就可增强MCF-7细胞形成细胞克隆的能力。结论邻苯二甲酸二丁酯可促进雌激素依赖性乳腺癌MCF-7细胞的增殖,可能具有拟雌激素作用。Objective To determine the estrogenic activity of dibutyl phthalate DBP. Methods The tested compound was dibutyl phthalate. Human estrogen-dependent MCF-7 breast cancer cells were cultured in RPMI 1640 medium containing 10% fetal bovine serum FBS. Five days before the addition of the tested compounds the cells were rinsed by phosphate balanced solution PBS and the medium was substituted with a phenol red-free RPMI 1640 medium containing 5% dextral charcoal-stripped FBS. The respective tested compound was added in fresh medium and the control cells received only the vehicle ethanol. The proliferation of MCF-7 cells was analyzed by the MTT assay growth curves mitotic index and coloning efficiency. Results Compared with the control the proliferation of tested cells treated with DBP like estradiol was markedly enhanced and the activity of the cell proliferation reached the maximum at 10-5 mol/L DBP. During log phase the mitotic index of the test cells treated with DBP and estradiol was significantly increased. The cell coloning efficiency was enhanced which was treated by 10-5 mol/L DBP only for 48 hours. The results showed the time-dependent and dose-dependent model. Conclusion Dibutyl phthalate may enhance the proliferation of human breast cancer MCF-7 cells in vitro that demonstrates an estrogenic activity of dibutyl phthalate.

关 键 词：雌激素类 邻苯二甲酸二丁酯 MCF-7细胞株 

分 类 号：R994.6[医药卫生—毒理学]