McAb　F（ab＇）_2免疫脂质体制备及其亲和性的放免测定  

Preparation of ￣125 I-F(ab')_2-DNR-liposomes and Radiotmmunoassay of Their Specific Conjugating Ability

作　　者：刘健[1] 陈正秋[1] 刘汉芝[1] 范启修[1] 沈德诚[1] 李树梅[1] 

机构地区：[1]中国医学科学院中国协和医科大学血液学研究所

出　　处：《中国医学科学院学报》1995年第5期383-386,共4页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金

摘　　要：ＭｃＡｂＦ（ａｂ＇）_２免疫脂质体制备及其亲和性的放免测定刘健，陈正秋，刘汉芝，范启修，沈德诚，李树梅（中国医学科学院，中国协和医科大学，血液学研究所，天津３０００２０）脂质体作为药物载体可降低药物毒性，提高药效［１，２］。由ＭｃＡｂ制备的兔疫脂质体?..Abstract The F(ab')2-DNR-liposomes were prepared using F(ab')2 obtained from digested monoclonal antibody against human white cells HI30(CD45).Their affinity constant for peripheral blood mononuclear cells was determined by radioimmunoassay. Ka=2.0×109 .A good specific conjugation ability was dernonstrated. In preparing the immunoliposomes,it was found that F(ab')2 coupled to the smaller liposomes with higher conjugation ratio.

关 键 词：免疫脂质体 制备 亲和性 McAb 

分 类 号：R392－33[医药卫生—免疫学]