免疫脂质体粒度与抗白细胞单抗F（ab＇）_2片段结合率关系的研究  

The Study of the Relationship between the Granule Size of Immunoliposomes and the Conjugate Ratio of F(ab')2 of McAb HI30(CD45)

作　　者：刘健[1] 刘汉芝[1] 陈正秋[1] 李树梅[1] 沈德诚[1] 范启修[1] 

机构地区：[1]中国医学科学院,中国协和医科大学

出　　处：《中国医学科学院学报》1995年第6期424-427,共4页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金

摘　　要：本实验采用^（１２５）Ｉ标记单抗ＨＩ_（３０）的Ｆ（ａｂ＇）_２片段，^（１３１）Ｉ标记脂质体膜的方法制备免疫脂质体，并观察其在Ｓｅｐｈａｃｒｙｌ－３００层析柱上的分离结果。证明超声法制备的脂质体粒度不均一，且粒度小的脂质体具有较高的单抗结合率。初步结果还显示，单抗Ｆ（ａｂ＇）_２片段与脂质体的偶联将明显增加免疫脂质体的粒度。The immunoliposomes were prepared by conjugating the F(ab')2 of monoclonal antibodies HI30(CD45) labelled by 125I with the liposomes labelled by 131I,and then were separated by a sephacryl S-300 column. The chromatography showed that the liposomes prepared by ultrasonic method were not homogeneous, and the liposomes of smaller granule size had a higher conjugate ratio. It was also found that the fragment F(ab')2 of McAb conjugated to liposomes could enlarge their granule size.

关 键 词：免疫脂质体 粒度 单抗结合率 

分 类 号：R392.1[医药卫生—免疫学]