异亚丙基莽草酸原料及其制剂抗栓素片的含量及稳定性研究  

Studies on determination of 3,4-O-isopropylidene-shikimic acid by HPLC and its stability in Kangshuansu tablet

作　　者：张桂燕[1] 马红飞[1] 李淑芝[1] 郭亚健[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《中国药学杂志》2005年第13期1020-1022,共3页Chinese Pharmaceutical Journal

基　　金：国家科技部"十五"科技攻关项目(2001BA701A07-14)

摘　　要：目的建立抗栓素片中异亚丙基莽草(ISA)的含量测定方法。方法采用RP-HPLC,Nova-pak-C18柱(3.9mm×150mm,4μm),流动相为乙腈-0.05%磷酸水溶液(15:85);检测波长:220nm;流速:0.8mL·min-1。结果异亚丙基莽草酸在0.1024-4.7268μg线性关系良好,r=0.9999(n=6),平均回收率为103.04%,RSD为1.21%(n=5)。结论本方法灵敏、准确、操作简便,重现性良好。OBJECTIVE: To establish a HPLC method for determining 3,4-O-isopropylidene- shikimic acid in Kangshuansu tablet. METHODS: A RP-HPLC separation was performed on a Nova pak-C18 column (3.9 mm × 150 mm,4 μm, Waerts, USA). The mobile phase was acetonitrile-0.05% phosphoric acid solution (15:85) at a flow rate of 0.8 mL&middotmin-1, and the detection wavelength was 220 nm. RESULTS: A good linearity for 3,4-O-isopropylidene-shikimic acid was obtained within the range of 0.102 4 ∼ 4.726 8 μg (r = 0.999 9), and the recovery was 103.04% (RSD = 1.21%, n = 5). CONCLUSION: The method is sensitive, accurate and simple, with a good reproducibility.

关 键 词：抗栓素片 异亚丙基莽草酸 稳定性 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]