中生菌素对水稻悬浮细胞苯丙氨酸解氨酶基因转录表达的影响  

The Transcript Accumulation of Phenylalanine Ammonialyase Gene in Rice Suspension-Cultured Cells Treated by Zhongshengmycin

作　　者：蒋细良[1] 朱昌雄[1] 王慧敏[2] 田云龙[1] 王劲波[1] 

机构地区：[1]中国农业科学院农业环境与可持续发展研究所,北京100081 [2]中国农业大学植物保护学院,北京100094

出　　处：《中国农业科学》2005年第7期1353-1357,共5页Scientia Agricultura Sinica

基　　金：国家自然科学基金项目(39970500);国家十五攻关计划项目(2001BA708B07);农业部生物防治资源评价与利用重点实验室项目

摘　　要：用白叶枯菌(HB84-17)、10μg·ml-1中生菌素和10μg·ml-1中生菌素+白叶枯菌处理水稻抗、感白叶枯病近等位基因系CBB4和沈农1033悬浮细胞,采用斑点杂交的方法研究了中生菌素对水稻悬浮细胞苯丙氨酸解氨酶基因转录表达的影响。结果表明,白叶枯菌、中生菌素和中生菌素+白叶枯菌处理都能诱导悬浮细胞中苯丙氨酸解氨酶基因的转录表达。对于CBB4,白叶枯菌处理3h时苯丙氨酸解氨酶基因开始诱导转录表达,6h达到高峰,诱导表达时间持续至48h;中生菌素处理3h时开始大量表达,大量表达持续至48h。对于沈农1033,白叶枯菌处理12h时苯丙氨酸解氨酶基因才开始诱导转录表达,诱导表达持续至24h;中生菌素处理,3h就开始大量转录表达,并持续至48h。中生菌素+白叶枯菌处理的感、抗品种苯丙氨酸解氨酶基因的诱导转录表达强度和模式与单用中生菌素处理相同。The transcript accumulation of phenylalanine ammonialyase gene of the suspension-cultured cells in the near-isogenic rice lines, which were treated by Zhongshengmycin, Xanthomonas oryzae pv. oryzae(Xoo), and Zhongshengmycin+ Xoo, respectively, was studied. The results showed that these different treatments could induce the transcript accumulation of phenylalanine ammonialyase gene in the rice suspension-cultured cells at different degrees. CBB4, the resistant rice line to Xoo, the transcript accumulation of the enzyme gene was induced at 3 h after treating with Zhongshengmycin, and inducing transcript lasted to 48 h in largely. Although the transcript accumulation was induced at 3 h after inoculating Xoo, reached the peak at 6 h and inducing transcript lasted to 48 h, the transcript accumulation was less. For Shennong 1033, the susceptible rice line to Xoo, the transcript accumulation of phenylalanine ammonialyase gene was induced from 3 h to 48 h after treating with Zhongshengmycin. While the inducing transcript was only accumulated at 12 h to 24 h after inoculating Xoo. The inducing degree and mode of transcript accumulation of phenylalanine ammonialyase gene treated with Zhongshengmycin+ Xoo were similar to Zhongshengmycin treatment.

关 键 词：中生菌素 转录表达 悬浮细胞 酶基因 酸解 苯丙 水稻 氨 白叶枯菌 近等位基因系 诱导表达 白叶枯病 斑点杂交 

分 类 号：S511[农业科学—作物学]