不同基因型孔雀草高效植株再生体系的建立  

The Establishment of Efficient Regeneration System for Different Genotypes of Tagetes patula L.

作　　者：齐迎春[1] 叶要妹[1] 刘国锋[1] 包满珠[1] 

机构地区：[1]华中农业大学园艺林学学院/教育部园艺植物生物学重点实验室

出　　处：《中国农业科学》2005年第7期1414-1417,共4页Scientia Agricultura Sinica

基　　金：国家"948"项目(2003-Z36)资助

摘　　要：将孔雀草品种T.patula‘LittleHeroGolden’的叶片、子叶和下胚轴接种于4种含有不同浓度IAA、NAA和BA的MS培养基上,比较不同外植体的再生植株的能力。结果表明,叶片再生植株能力较子叶和下胚轴强。随后,进一步将其叶片接种于9种添加不同浓度NAA和BA的MS培养基上,分化结果显示,在MS+NAA1.5mg·L-1+BA1.0mg·L-1的培养基上植株再生率最高,达75%。5份材料的叶片分别接种在该培养基上,再生率均达70%以上,其中T.patula21614的再生率达85%。所有再生植株均能成苗并表现出正常的形态特征。从而建立了适合于5种基因型孔雀草的离体高效再生体系。In order to test the regeneration ability of different explants, the leaves, cotyledons and hypocotyls of T. patula ‘Little Hero Golden’ were cultured on 4 kinds of media, MS + NAA 0.5 mg·L-1+ BA 1.0 mg·L-1, MS + NAA 1.0mg·L-1 + BA 2.0 mg·L-1, MS + NAA 1.5 mg·L-1 + BA 2.0 mg·L-1 and MS + IAA 3.0 mg·L-1 + BA 2.5 mg·L-1. The results showed that the regeneration ability of leaves was higher than that of cotyledons and hypocotyls. The leaves were then cultured on 9 kinds of MS media with different combinations of NAA and BA, and the highest regeneration frequency was obtained on MS medium supplemented with 1.5 mg·L-1NAA and 1.0 mg·L-1 BA, and the regeneration rate was up to 75%. The medium was tested for other 4 genotypes, and all the lines were successfully regenerated with frequencies from 70% to 85%. All the regenerated plantlets developed into complete plants of normal morphological characteristics.

关 键 词：植株再生体系 孔雀草 基因型 MS培养基 Little 再生植株 高效再生体系 植株再生率 形态特征 下胚轴 NAA 叶片 IAA 接种 外植体 浓度 子叶 BA 能力 添加 成苗 

分 类 号：S682[农业科学—观赏园艺]