长白山温泉中一株新高温菌的筛选及其特征  

A Novel Thermophilic Bacterium Isolated from a Hot Spring in Chang-bai Mountains and Its Characteristic of Morphology

作　　者：高朝辉[1] 孟庆繁[1] 逯家辉[1] 冯德日 蒋朝军[1] 陈亚光[1] 滕利荣[1] 

机构地区：[1]吉林大学生命科学学院,长春130012

出　　处：《吉林大学学报（理学版）》2005年第4期527-532,共6页Journal of Jilin University:Science Edition

基　　金：全国教育科学"十五"规划项目基金(批准号:DIA030165);吉林省教育科学"十五"规划项目基金(批准号:B315041).

摘　　要：从长白山上一个温泉周围的土壤中分离、纯化出一株高温菌CW2. 分离出的菌株CW2为革兰氏阳性, 在生长过程中细菌细胞的形态有球杆的变化, 在对数生长期的初期细胞是直杆或者弯杆状, 大多数单生, 少数成对或者成不连续的链状, 到恒定期变成类球形细胞. 菌株CW2有芽孢、荚膜、鞭毛、运动性, 无伴胞晶体, V.P.实验呈阴性, 能降解淀粉, 不能降解纤维素, 兼性好氧, 生长的pH值为5～11、温度30～60 ℃; 最适生长pH值为7.5、温度55 ℃. 菌落初形成时是乳白色边缘整齐的圆形, 后来就会随机向3个方向发展, 最终形成不规则圆形菌落、花瓣形菌落或者扁平扩散形菌落.A thermophilic strain (CW_2) was isolated and purified from the soil around a hot spring in Chang-bai mountains. The cells of isolate (CW_2) stain Gram positively, the morphology of the cells of isolate was changed from rod to round in the process of growth, the cells in the initial phase of logarithmic growth were long rod, majority of cells singly existed, minority of cells were geminated or formed a long discontinuous chain, until to invariable phage of growth, the cells changed to globe. Strain (CW_2) formed spore, had capsule, flagella, (motility,) no parasporal crystal, V.P. test was negative, could catabolism starch, could not catabolism fibrin, (aerobic,) but could grow without oxygen, grow at and between 3060 (opt. 55 ), grow at and between pH 5~11(opt. pH 7.5). The neonatal colonies were milk-white and round and the margin is regular, (growing) along with the strain (CW_2), the colonies randomly developed to three shapes, finally formed irregular round colonies, flowery colonies or radiate compressed colonies.

关 键 词：长白山温泉 高温菌 分离纯化 特征研究 

分 类 号：Q93-331[生物学—微生物学]