17-β-雌二醇对凝血酶诱导血管平滑肌细胞增生和c-fos、bcl-2蛋白表达影响  

Effects of 17-β-Estradiol on Proliferation of Vascular Smooth Muscle Cells Induced by Thrombin and c-fos,Bcl-2 Expression

作　　者：许逸洲[1] 王宁夫[1] 李佩璋[1] 徐海鹰[1] 徐坚[1] 

机构地区：[1]浙江省杭州市第一人民医院心血管内科,310006

出　　处：《医学研究通讯》2005年第7期22-25,共4页Bulletin of Medical Research

摘　　要：目的通过建立凝血酶(T)诱导的血管平滑肌细胞(VSMC)增生模型,观察17-β-雌二醇(E_2)对 T 诱导的VSMC 增生的影响,并进一步观察其对 c-fos 蛋白和 bcl-2蛋白的表达有何作用,旨在认识 E_2作用的分子机制。方法以细胞计数法和流式细胞仪 DNA 含量测定,细胞周期分析的方法观察 T 及 E_2对 VSMC 增生和 DNA 合成的影响。免疫印迹法(Western blot)观察 T 及 E_2等各处理因素作用1小时及3天后,c-fos 蛋白和 bcl-2蛋白的变化情况。结果 10^(-9)～10^(-7)M 的E_2呈浓度依赖性地抑制2u/ml T 诱导的细胞增生和 DNA 合成作用(P<0.01)。各处理因素作用1小时后,T 能显著促进 c-fos蛋白的增加。各浓度的 E_2能显著抑制 T 的促 c-fos 蛋白增加作用,作用3天后,c-fos 蛋白含量各组差异无显著性。各处理因素作用1小时后,bcl-2蛋白无显著变化,作用3天后,T 能显著促进 bcl-2蛋白的增加。各浓度的 E_2能显著抑制这种增加。上述各项指标中,雌激素受体(ER)拮抗剂 tamoxifen(TAM)(10^(-6)M)能部分阻断10^(-7)M E_2的作用,差异有显著性。结论 E_2能抑制 T 诱导的 VSMC 增生,这可能与其抑制 c-fos 蛋白和 bcl-2蛋白表达有关,且该作用至少部分通过 ER 介导。Objective To observe the role of puerarin on the proliferation of vascular smooth muscle cells(VSMC)induced by thrombin and the effect of puerarin on the c-fos protein and bcl-2 protein expression.Methods Cell number and cell cycle analysis using flow cytometry were adopted as two different indicators of effects on proliferation of VSMC.Western blot was used to indicate the changes of c-fos protein and bcl- 2 protein with treatment of T and E_2 after 1 hour and 3 days.Results 10^(-9)～10^(-7) M E_2 can significantly suppress VSMC proliferation and DNA synthesis induced by 2u/ml T.T can significantly increase c-fos protein treated after 1 hour,E_2 can significantly suppress this increase;treated after 3 days,the difference of c-fos protein in each group was not significant,bcl-2 proteins were of no significant difference after treated 1 hour,but after 3 days,T can significantly increase bcl-2 protein,E_2 can suppress this increase.All indicators proved that 10^(-6) M TAM can partly inhibit the effects of 10^(-7) M E_2.Conclusions E_2 can suppress the proliferation and DNA synthesis of VSMC promoted by T.This inhibitory ef- fects of E_2 are closely related with the suppression of c-fos protein and bcl-2 protein,which is,at least,partly mediated by ER.

关 键 词：bcl-2蛋白表达 血管平滑肌细胞增生 17-Β-雌二醇 C-FOS蛋白 酶诱导 VSMC增生 雌二醇(E2) DNA合成作用 细胞周期分析 处理因素 流式细胞仪 细胞计数法 免疫印迹法 浓度依赖性 雌激素受体 分子机制 含量测定 变化情况 蛋白含量 

分 类 号：R977.11[医药卫生—药品] R543[医药卫生—药学]