细胞外基质受体α-Dystroglycan影响胸腺细胞分化发育的可能机制  

Mechanisms of extracellular matrix receptor α-Dystroglycan on thymocytes differentiation

作　　者：张瑞华[1] 张进平[1] 何叶成[1] 邵先安[1] 龚燕萍[1] 苏丽萍[1] 熊思东[1] 

机构地区：[1]复旦大学上海医学院免疫系

出　　处：《中华微生物学和免疫学杂志》2005年第6期438-441,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金项目(30471569);上海联合利华研究与发展基金资助项目(KEF120808);上海市优秀学科带头人计划资助(04XD14003)

摘　　要：目的研究细胞外基质受体alphaDystroglycan(α-DG)对胸腺细胞分化的影响及机制。方法摘取15日胚龄鼠胸腺小叶进行体外器官培养。将α-DG抗体、对照抗体或培养液滴加在胸腺小叶上。FACS(Fluorescenceactivatedcellsorting)分析胸腺细胞表面分子CD4、CD8、CD95和CD69等的表达。结果α-DG中和抗体能明显抑制胸腺细胞分化,显示胸腺双阴性细胞比例从对照组的26.5%增高到实验组的71.6%,双阳性细胞和CD8单阳性细胞比例则显著下降,分别从39.8%和20.7%下降到7.5%和6.8%,CD4单阳性细胞比例则无明显变化;同时胸腺细胞数目明显减少;CD95、CD69的表达水平随α-DG中和抗体的持续存在呈现显著升高。结论α-DG通过参与胸腺细胞的活化和凋亡活动影响胸腺细胞的发育。Objective To investigate the effect and mechanism of extracellular matrix receptor α-Dystroglycan(α-DG) on thymocytes differentiation in vitro. Methods The thymus lobe of fetal mice was cultured in FTOC, and the α-DG McAb, isotype Ab or medium was added into the culture respectively. The expression of CD4, CD8, CD95 and CD69 of fetal mice thymus lobe were examined by FACS. Results α-DG McAb inhibited the differentiation of thymocytes. The rate of DN thymocytes markedly increased from 26.5% in control to 71.6% in the group with α-DG McAb after 6-day culture. DP and CD8+ SP cells decreased obviously from 39.8% and 20.7% to 7.5% and 6.8%. But the rate of CD4+ SP cells did not change. The number of thymocytes also decreased after α-DG McAb neutralization. Meanwhile, the expression of CD95 and CD69 in thymocytes remarkably increased. Conclusion Dystroglycan participates in the thymocytes differentiation partly dependent on interference thymocytes activation and apoptosis.

关 键 词：细胞外基质 可能机制 分化发育 受体Α 胸腺细胞分化 单阳性细胞 体外器官培养 细胞表面分子 CD69 CD95 双阳性细胞 cell 阴性细胞 细胞数目 持续存在 中和抗体 CD4 CD8 培养液 实验组 对照组 比例 小叶 下降 

分 类 号：R392[医药卫生—免疫学]