四川西北地区川西獐牙菜指纹图谱研究(Ⅰ)  

Study on HPLC fingerprint of Swertia mussotii var.mussotii from north-west area of Sichuan province Ⅰ

作　　者：吉文鹤[1] 卢学峰[1] 陈桂琛[1] 纪兰菊[1] 

机构地区：[1]中国科学院西北高原生物研究所,青海西宁810001

出　　处：《中国中药杂志》2005年第13期977-979,共3页China Journal of Chinese Materia Medica

基　　金：国家科技攻关计划项目(2001BAg01A47)青海省重点科技攻关项目(2004-N-142)

摘　　要：目的:建立川西獐牙菜药材甲醇溶解部分的HPLC指纹图谱。方法:反相高效液相色谱法,流动相甲醇.水(水中含0.02%的磷酸),梯度洗脱,流速1.0mL·min-1,检测波长260nm,柱温30℃。结果:精密度、重复性、稳定性试验中共有峰峰面积、保留时间的RSD均小于5.0%;不同产地川西獐牙菜的相似度大于0.805。结论:该方法简便、实用、可靠,可作为川西獐牙菜药材的质量标准。Objective: To constitute HPLC fingerprint of the methanol extract from Swertia mussotii grown in Sichuan Province. Mxethod: RP-HPLC, methanol and water including 0.02% acid as mobile phase, gradient elution, flow rate 1.0 mL·min-1 ,the detection wavelength was 260 nm, temperature was 30 ℃. Result: The RSD values of peak area and retention time of common peaks in precision, repeatability and stability were lower than 5.0%, respectively, similarity was over 0.805 in S. mussotii collected from 10 different habitats. Conclusion: All results above exhibit that this method is simple, practicable, and reliable as a standard method in controlling the quality of S. mussotii.

关 键 词：川西獐牙菜 反相高效液相色谱法 指纹图谱 质量控制 

分 类 号：S567.2[农业科学—中草药栽培]