二仙汤及其拆方对大鼠性腺细胞分泌功能的影响  

Effects on secretory function of rat gonad by erxian decoction and its disassembled prescriptions

作　　者：刘晴[1] 施建蓉[1] 杨颖 方肇勤[1] 梁尚华[1] 郭瑞新[1] 

机构地区：[1]上海中医药大学,上海201203 [2]上海市内分泌研究所,上海200025

出　　处：《中国中药杂志》2005年第13期1023-1026,共4页China Journal of Chinese Materia Medica

基　　金：教育部优秀青年教师资助计划(1632)

摘　　要：目的:观察二仙汤及其温肾、滋阴、调理冲任3个拆方对性腺细胞分泌功能的影响,比较二仙汤3个组成部分对性腺细胞的作用、差异和协同,从细胞水平揭示其配伍组方的原理。方法:按方剂组成原理进行拆方,用放射免疫方法观察二仙汤及其拆方对原代培养雄性大鼠睾丸间质细胞分泌睾酮(T)、原代培养雌性大鼠黄体细胞孕酮(P)及卵泡颗粒细胞分泌雌二醇(E2)的影响。结果:①对睾丸间质细胞分泌T的作用,以二仙汤全方组促进作用最明显,而滋阴组有降低T的趋势,温肾组和当归组作用不明显;②对黄体细胞分泌P的作用,以全方组促进作用最好,其次为温肾组,当归组和滋阴组的作用不明显;③对促进颗粒细胞分泌E2的作用,以全方组效果最好,其次为温肾组、滋阴组,而当归有提高E2的趋势。结论:二仙汤能直接促进睾丸间质细胞分泌T,黄体细胞分泌P以及促进颗粒细胞分泌E2,综合来看,全方组作用最好,拆方组作用有异同。Objective: To explain functions, differences and coordination of three divided combinations of the 'Erxian decoction', the famous traditional Chinese formula, on the effective sites of gonad gland at the cell level. Method: The effects of Erxian decoction and its main disassembled prescriptions, 'Kidney Warming', 'Yin Nourishing' and 'Chong-ren Adjusting', on the level of testosterone (T) progesterone (P) estradiol (E2), respectively secreted by the primary culture leydig cell, luteal cell and granulosa cell, were measured by radioimmunoas say. Result: (1) Erxian decoction could stimulate the T secretion while its three main disassembled prescriptions would seem no individual promoting effect on the secretion of T. (2) Erxian decoction and the 'Kidney Warming' had the stimulating effect on psecretion, and the action of the whole formula being better than that of the 'Kidney Warming'. (3) Erxian decoction and its main disassembled prescriptions had the stimulating effect on E2 secretion, especially the whole formula. Conclusion: Erxian decoction can stimulate the secretion of T of the leydig cell, P of luteal cell and E2 of granulosa cell. It can be seen that the effect of the whole formula is better than that of its main disassembled prescriptions.

关 键 词：二仙汤 中药复方 拆方 雌二醇 孕酮 睾酮 放射免疫测定 

分 类 号：R285.5[医药卫生—中药学]