反相高效液相色谱法同时测定洁康舒洗剂中三组分的含量  

Determination of Rhen,Emodin and Chrysophanol in Jiekangshu Iotion by RP-HPlG

作　　者：刘腊娥[1] 陈立新[1] 魏萍[2] 王绪明[2] 

机构地区：[1]中国人民解放军第163医院药剂科,湖南长沙410003 [2]中国人民解放军总后勤部卫生部药品仪2S检验所,北京100071

出　　处：《福建分析测试》2005年第1期2101-2102,2111,共3页Fujian Analysis & Testing

摘　　要：建立反相高效液相色谱法同时测定洁康舒洗剂中大黄酸、大黄素和大黄酚含量的方法.采用反相高效液相色谱法.symmctry C18柱(3.9×150mm,5μm)流动相为甲醇-水-磷酸(80:20:0.1);流速为1.OmL·min-1;检测波长为432nm.大黄酸、大黄素和大黄酚的回归方程分别为:C1=7.3159×10-1+1.8966×10-5A,r=9994,C2=1.0513+1.5680×10-5A,r=0.9999 C3=1.2260-1.4684×10-5A,r=0.9999三者分别在7.60～38.0μg·mL-1、1.75～8.75μg·mL-1、2.4～12.00μg·mL-1范围内呈线性关系.平均回收率和RSD分别为98.02%、96.63%、97.05%和1.78%、1.20%、1.15%.该方法操作简便,测定结果准确可靠,可用于测定洁康舒洗剂中大黄酸、大黄素和大黄酚的含量.A quantitative method was developed for the determination of Rhen,Emodin and Chrysophanol in jiekangshu lotion by RP—HPLC.The chromatographic conditions includ column Symmetry C_ 18,mobile phase: methanol-water-phosphoric acid(80∶20∶0,1),flow rate 1.0 mL·min -1,detection wavelength 432nm.The regression equation of Rhenm,Emodin and Chrysophanol was C_1=7.3159X10 -1+1.8966×10 -1A,r=0.9994;C_2 =1.0513+1.5680X10 -5A,r=0.9999;C_3=1.2260-1.4684×10 -5A,r=0.9999,respectively The standard curve was linear in the range of 7.60～38.0 μg·mL -1, 1.75～8.75 μg·mL -1 and 2.40～12.00 μg·mL -1, respectively.The average added recovery and correlation coefficient were 98.02%、96.63%、97.05%and 1.78%、 1.20%、1.15%,respectively. The method was simple, sensitive,rapid and accurate. It may be used fOr determination of three component in jiekangshu lotion.

关 键 词：反相高效液相色谱法 同时测定 洗剂 三组分 平均回收率 大黄酸 大黄素 C18柱 检测波长 回归方程 线性关系 测定结果 大黄酚 酚含量 醇-水 流动相 RSD 

分 类 号：O657.72[理学—分析化学] O657.32[理学—化学]