反相高效液相色谱法测定肾康口服液中黄柏的小檗碱含量  

Determination on berberine of Huangbo in Shenkang oral liquid by RP—HPLC

作　　者：刘腊娥[1] 陈立新[1] 梁爱君[2] 王绪明[2] 

机构地区：[1]中国人民解放军第163医院药剂科,湖南长沙4100032 [2]中国人民解放军总后勤部药品仪2S检验所,北京100071

出　　处：《福建分析测试》2005年第1期2123-2125,共3页Fujian Analysis & Testing

摘　　要：建立反相高效液相色谱法测定肾康口服液中黄柏的小檗碱含量.采用反相高效液相色谱法,Novapak C18柱(3.9×150mm,5μm)流动相为甲醇-乙腈(0.05mol·L-1)-磷酸二氢钠溶液(20:3S:45),流速为1.0mL·min-1,检测波长为346nm.小檗碱的回归方程为C=1.2616+1821X10-6A,r=0.9992,在4.00～20.00μg·mL-1范围内呈线性关系.平均回收率和RSD分别为101.32%和1.18%(n=5).方法操作简便,测定结果准确可靠,可用于测定肾康口服液中黄柏的小檗碱含量.A quantitative method was developed for the determination on berberine of Huangbo in Shengkang oral liquid by RP-HPLC.Thechromatographic conditions include column Novapak C_ 18(3.9×150mm,5μn),mobile phase:methanol-methylcyanide(0.05mol·L -1-sodium dihydrogen phosphate(20∶35∶45),flow rate1.omL/ min,detection waVelength 346nm.The regression equation of berberine hydrochloride was C=1.2616+8.1821×10 -6A r-0.9992. The standard curve was linear in the range of 4.00～20.00 μg·mL -1. The average added recovery and RSD were 101.32%and 1.18% (n=5),respeetively. The method was simple sensitive,rapid and accurate.It may be used for determination of berberine in Huanbo of shenkang oral liquia.

关 键 词：反相高效液相色谱法 碱含量 口服液 黄柏 肾 磷酸二氢钠 平均回收率 C18柱 检测波长 回归方程 线性关系 测定结果 流动相 小檗碱 RSD 溶液 

分 类 号：O657.72[理学—分析化学] TU528.042[理学—化学]