纯化眼镜蛇毒蛋白对乙酰胆碱受体通道的阻断作用  

BLOCKING EFFECT OF VENOM FROM CORA ON ACETYLCHOLINE RECEPTOR CHANNELS

作　　者：董岩[1] 汤云贵[1] 蒋伍玲[1] 孙志峰[1] 黄颖[1] 谢佐平[1] 覃志忠[1] 

机构地区：[1]清华大学生物科学与技术系,沈阳生物资源医用研究所

出　　处：《中国应用生理学杂志》1995年第2期113-116,共4页Chinese Journal of Applied Physiology

摘　　要：在培养的爪蟾胚胎神经和肌肉细胞上用膜片箝技术研究了从中国华南产眼镜蛇毒分离出的神经毒组分对乙酰胆碱受体（ＡＣｈ－Ｒ）通道的影响，发现它在微终板电位和单通道水平上都有明显的阻断作用。完全阻断神经肌肉接点传递的剂量约为２０．μｇ／ｍｌ，对乙酰胆碱受体通道的半阻断量约为０．２３μｇ／ｍｌ。这表明它与α－银环蛇毒有相似功效。Nerve toxin (Peak-9 cobra toxin,P9-CTX)is isolated from the venom of Chinese cobra.Patch clamp technique is first used to study the inhibitory effect of this toxin on acetylcholinereceptors on the membrane of innervated muscles. By whole-cell clamp and on-cell clamp,it wasfound that there was obvious inhibitory effect of this toxin on both miniature end-plate potential(MEPP) and single acetylcholine receptor (ACh-R)channel.The minimal dose for complete in-hibition of MEPP was about 2.0μg / ml.The dose for 50% inhibition of single channel was about0.23μg / ml.These indicate the similarity between -bungarotoxin and this toxin.

关 键 词：眼镜蛇毒 乙酰胆碱受体 毒蛋白 

分 类 号：R996.3[医药卫生—毒理学]