降钙素基因相关肽对大鼠分离的心肌细胞内游离Ca^（2＋）含量的影响  

EFFECT OF CALCITONIN GENE RELATED PEPTIDE ON INTRACELLULAR FREE Ca￣(2+) CONTENT IN ISOLATED RAT CARDIAC MYOCYTE

作　　者：徐春[1] 孙明智[1] 李玉荣[1] 杨宝峰[1] 

机构地区：[1]哈尔滨医科大学生理学教研室,哈尔滨医科大学药理学教研室

出　　处：《中国应用生理学杂志》1995年第3期244-246,共3页Chinese Journal of Applied Physiology

摘　　要：用荧光染色法观察了合成的大鼠降钙素基因相关肽（ＣＧＲＰ）对大鼠心肌细胞内游离Ｃａ￣（２＋）含量的影响。结果证明，ＣＧＲＰ能明显增加心肌细胞内Ｃａ￣（２＋）含量，小、中和大剂量（１０￣（－９）、１０￣（－８）、１０￣（－７）ｍｏｌ／Ｌ）的ＣＧＲＰ使Ｃａ￣（２＋）含量分别增加至２７６．８８±６．３１、３６４．９９７±１２．７０、５７６．３９７±１５ｎｍｏｌ／Ｌ与对照组（１３６．２８±７．２４ｎｍｏｌ／Ｌ）相比差异非常显著（Ｐ＜０．０１），且随着ＣＧＲＰ剂量的增加而作用明显加强，呈现剂量—效应关系。３０μｍｏｌ／Ｌ的维拉帕米对ＣＧＲＰ所致的细胞内Ｃａ￣（２＋）增加有抑制作用，对小、中、大剂量ＣＧＲＰ作用的抑制率分别为４８％、４４％和１８％。我们推测，ＣＧＲＰ可能直接作用于心肌细胞。低浓度ＣＧＲＰ的正性肌力作用主要是促进Ｃａ￣（２＋）经Ｃａ￣（２＋）通道内流，使心肌细胞内Ｃａ￣（２＋）含量增加的结果。在大剂量ＣＧＲＰ的正性肌力作用中Ｃａ￣（２＋）内流也起到一定作用。The effect of synthetic rat CGRP on intracellular free Ca￣(2+) content was observed in the iso-lated rat cardiac myocyte. The results showed that CGRP increased significantly intracellularCa￣(2+) content from 136.28 ± 7.24nmol/L in the control to 276.88 ± 6.31. 365.00 ± 12.70 and576.40± 15nmol/ L in small(10￣(-9) mol/ L),medium(10￣(-8)mol/L)and large CGRP dosage(10￣(-7)mol / L)groups respectively with a dose─dependent manner. When verapamil(30μmol / L)was given simultaneously with CGRP at the three dosages mentioned above, the increased Ca￣(2+)content induced by CGRP was depressed.The rates of depression of Ca￣(2+) at small and mediumdosages of CGRP were 48%, 44% respectively,which were significantly higher than that in largeCGRP dosage (18%).It is,therefore,considered that the positive inotropic action of CGRP atlow concentration is the result of enhancing influx of Ca￣(2+)into the cell through Ca￣(2+) channel.The influx of Ca￣(2+) also plays a certain role in the positive inotropic action of CGRP at highdosage.

关 键 词：降钙素基因 相关肽 心肌细胞 游离钙 CGRP 

分 类 号：R331.31[医药卫生—人体生理学] Q516[医药卫生—基础医学]