培养乳鼠心肌细胞自发性〔Ca^（2＋）〕_i瞬间变化的实验模型  

EXPERIMENTAL MODEL FOR STUDYING SPONTANEOUS [Ca￣(2+)]_ i TRANSIENTS IN CULTURED MYOCARDIAL CELLS OF NEONATAL RATS

作　　者：王洪新[1] 陶亮[1] 饶曼人[1] 王金唏[1] 杨思军[1] 

机构地区：[1]南京医科大学心血管药理学研究室,南京大学配位化学国家重点实验室,锦州医学院药理学教研室

出　　处：《中国应用生理学杂志》1995年第4期368-371,共4页Chinese Journal of Applied Physiology

摘　　要：使用Ｃａ￣（２＋）敏感的荧光指示剂Ｆｕｒａ－２／ＡＭ，对培养的心肌细胞测定细胞内Ｃａ￣（２＋）的瞬间变化。结果为，在一个心搏周期中，经过大约１８０ｍｓ的时间，［Ｃａ￣（２＋）］＿ｉ瞬间变化达到最大值，然后恢复到基线水平（最小值），经历了２６０ｍｓ。测得平均［Ｃａ￣（２＋）］＿ｉ瞬间变化的最大值及最小值分别为３４６±３８ｎｍｏｌ／Ｌ和１０２±１８ｎｍｏｌ／Ｌ。血管紧张素Ⅱ１００ｎｍｏｌ／Ｌ引起［Ｃａ￣（２＋）］＿ｉ瞬间变化最大值明显增加，但对［Ｃａ￣（２＋）］＿ｉ瞬间变化的基线水平没有明显的影响。ｒｙａｎｏｄｉｎｅ３μｍｏｌ／Ｌ使［Ｃａ￣（２＋）］＿ｉ瞬间变化的幅度明显降低。ＫＣｌ５０ｍｍｏｌ／Ｌ使［Ｃａ￣（２＋）］＿ｉ瞬间变化完全停止，并明显增加［ｃａ￣（２＋）］＿ｉ的基线水平。结果提示，本实验模型可用来观察［Ｃａ￣（２＋）］＿ｉ的瞬间变化。By using Ca￣(2+)-sensitive fluorescent indicator Fura-2/AM,spontaneous cytosolic freeCa￣(2+)transients were measured in cultured myocardial cells of neonatal rats.During a cardiac cy-cle,[Ca￣(2+)]_i transients reached a maximum at about 180 ms,then returned to basal level overthe following 260 ms.Average end-diastolic(minimal) and peak-systolic(maximal)[Ca￣(2+)]_iwere 102 ± 18 nmol / L and 346 ± 38 nmol / L,respectively. Treatment of the myocytes withangiotensin Ⅱ 100 nmol / L caused an increase in the peak of Ca￣(2+)transients with no apparentchange in the basal[Ca￣(2+)]_i level between beats.Ryanodine 3 μ mol/ L markedly inhibited themaximal levels of [Ca￣(2+)]_i transients.KCl 50 mmol / L completely blocked[Ca￣ (2+)]_i transi-ents and significantly increased basic level of[Ca￣ (2+)]_i. The results suggest that this experimentalmodel may be useful for observing the[Ca￣(2+)]_i transients in cultured myocardial cells.

关 键 词：心脏功能 心肌细胞 钙 实验模型 

分 类 号：R331.31[医药卫生—人体生理学]