24味中药对人肺腺癌细胞核酸和蛋白质及细胞周期的影响观察  

Effects of 24 Chinese Medicinal Herbs on Nucleic Acid, Protein and Cell Cycle of Human Lung Adenocarcinoma Cell

作　　者：韩明权[1] 刘嘉湘[1] 高虹[1] 朱晏伟[1] 陈善香[1] 

机构地区：[1]上海中医药大学附属龙华医院

出　　处：《中国中西医结合杂志》1995年第3期147-149,共3页Chinese Journal of Integrated Traditional and Western Medicine

摘　　要：运用流式细胞仪，对临床治疗原发性肺癌的有效复方进行了拆方研究，通过观察太子参等２４味药物作用后人肺腺癌ＳＰＣ－Ａ－１细胞增殖指数、脱氧核糖核酸（ＤＮＡ）指数、蛋白质指数和细胞周期各时相比例的变化等指标，部分揭示了临床有效复方治疗肺癌的作用机理。同时发现其中对增殖指数抑制率在２０％以上的４味药中，有３味是扶正药（绞股蓝、北沙参、人参），提示我们用扶正法治疗肿瘤并不一定意味着传统意义上的“补”，一些补益药亦能直接抑制肿瘤细胞增殖，即“祛邪”。并发现２４味单味药中对人肺腺癌ＳＰＣ－Ａ－１细胞周期作用点各不相同的一些药物，进而可以根据实验结果重组有效复方运用于临床，或根据中西药物各自特点，与化疗药物有机结合，可望能更有效地阻滞癌细胞增殖。Using flow cytometry the authors analysed the effect of 24 Chinese medicinal herbs (CMH)in compound recipe on proliferation index (PI), DNA index,protein index and ratio of various phases in cell cycle of human lung adenocarcinoma cell (SPC-A-1), The PI were more than 20%, in 4 CMH,while 3 CMH such as Gynostemma pentaphyllum, Glehnia littoralis, Panax Ginseng,could strengthen the body resistance.That suggested using CMH of strengthening body resistance not only served as conventional tonice but also as tumor cell inhibitor. Meanwhile the action point of 24 CMH on cell cycle were different. Therefore according to these results a new recombined Chinese recipe would be more effectively used for clinical practice.

关 键 词：中药 扶正中药 细胞周期 核酸 肺肿瘤 腺癌 

分 类 号：R273.420.5[医药卫生—中西医结合] R285.5[医药卫生—中医肿瘤科]