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作 者:KaiSUN GangLIU Yun-xuanZHANG Ghen-liangYIN Gaj-junTIAN Jia-huPAN
机构地区:[1]DepartmentofPharmacology,SchoolofPharmacy,FudanUniversity,Shanghai,200032
出 处:《中国药理通讯》2005年第2期37-38,共2页
摘 要:AIM To study the protective effects of the new standardized preparation of Ginkgo biloba extracts(GBE50) anainst brain mitochondrial damages induced by hyperlipemia in rats. METHODS Rat model with hyperlipemia was established by feeding the young male SD rats (3 weeks after born) with high lipid food for 3 months. Then the rats were treated with different dosage of GBE50(ig) for 4 weeks. The prophylaxis rat group were fed with the mixture of high lipid food and GBE50 (50mg/kg/d). The brain mitochondria was separated for determination of the R3, R4 and RCR of the respiration function with the method of Clark's electrode. The mitochondrial transmembrane potential(Δψm) was derected by the Rho123 assay and the cytochrome c release was checked by spectrography. In addition, the parameters of oxidative stress (the activities of SOD, GSH-Px, and the MDA leve) and the activities of ATPase were assayed.
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