中国人神经珠蛋白(NGB)基因克隆与序列分析  

Cloning and Sequencing of Chinese Neuroglobin(NGB)Gene

作　　者：牛建章[1] 田侠[1] 赵慧[1] 叶步青[2] 廖祥儒[1] 赵晓瑜[1] 

机构地区：[1]河北大学生命科学学院,河北保定071002 [2]中国科学院上海生物工程研究所,上海200000

出　　处：《河北大学学报（自然科学版）》2005年第4期412-417,共6页Journal of Hebei University(Natural Science Edition)

基　　金：河北省科技厅攻关项目(3276101)

摘　　要：应用RT_PCR方法从中国人胎脑组织中扩增出神经珠蛋白(NGB)特异性编码基因片段,并将其克隆到pMD18_TVector中,构建了pMD18_T_hNGB克隆质粒,然后进行测序分析并与国外报道的NGB序列相比较.结果表明,本文获得的中国人NGB编码基因序列与国外序列的同源性为98%.Using the method of RT-PCR, neuroglobin(NGB) specific gene fragment from human brain tissue was amplified, then it was cloned to pMD 18_T Vector. The pMD18_T_hNGB cloning plasmid were constructed. The gene fragment was sequenced and compared with NGB sequence reported by foreigners.The results indicated that the homogeneity between Chinese NGB gene and the gene from abroad is 98%.The findings build up important foundation for study of expression, biological function of Chinese NGB gene, and for developing new bioengineering medicines.

关 键 词：人胎脑 神经珠蛋白(NGB) 基因 克隆 序列分析 

分 类 号：Q523.8[生物学—生物化学]