β-环糊精修饰区带毛细管电泳法测定野菊花中刺槐素、木犀草素和槲皮素  

DETERMINATION OF ACACETIN LUTEOLIN AND QUERCETIN IN CHRYSANTHEM INDICUM L BY β-CYCLODEXTRIN MODIFIED CAPILLARY ZONE ELECTROPHORESIS

作　　者：吕元琦[1] 李新民[1] 王月伶 袁倬斌[2] 

机构地区：[1]德州学院化学系,德州253023 [2]中国科学院研究生院应化所,北京100049

出　　处：《理化检验（化学分册）》2005年第7期464-466,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：国家自然科学基金(20175025;29875027;20235010)资助项目

摘　　要：通过研究缓冲溶液pH和浓度、工作电压、SDS浓度和-βCD浓度等条件,建立了β-环糊精修饰区带毛细管电泳法测定野菊花中刺槐素、木犀草素和槲皮素的方法。在优化的条件下,3种物质在8min内得到良好分离。刺槐素、木犀草素和槲皮素峰面积和质量浓度分别在2~400,2~500和2~500mg.L-1范围内呈良好线性,检出限分别为0.6,0.5和0.6mg.L-1。该方法被用于分析实际样品并得到满意的结果。A method was developed to determine acacetin, luteolin and quercetin in Chrysanthem indicum L by β-CD modified capillary zone electrophoresis. The pH and concentration of buffer, applied voltage, concentration of SDS and β-CD concentration were optimized. Under the optimized conditions. The 3 analytes can be well separated in 8 min. A good linear relationship between the peak area and concentration was found in the ranges of 2~400, 2~500 and 2~500 mg·L^(-1) for acacetin, luteolin and quercetin respectively. The relative standard deviation for migration time and for peak area were 0.51%, 0.76%, 0.83% and 2.17%, 2.42%, 2.68% for acacetin, luteolin and quercetin respectively. The detection limits (S/N=3) were 0.6, 0.5 and 0.6 mg·L^(-1) for acacetin, luteolin and quercetin respectively. The method has been applied to the analysis of some samples and the accuracy verified by the recovery test.

关 键 词：区带毛细管电泳(CZE) β-环糊精(β-CD) 野菊花 刺槐素 木犀草素 槲皮素 

分 类 号：O657.17[理学—分析化学]