性激素对抗体分泌细胞表面分子表达和抗体分泌的影响  

Effects of sex hormones on expression of surface molecular markers on antibody-secreting hybridoma cells and on antibody production

作　　者：俞明灿[1] 王晓蕾[2] 许金菊[2] 曹筱梅[2] 贲昆龙[2] 

机构地区：[1]中国科学院研究生院,北京100039 [2]中国科学院昆明动物研究所分子与细胞免疫学实验室,昆明650223

出　　处：《中国免疫学杂志》2005年第7期487-491,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金资助项目(30170894)

摘　　要：目的:探讨性激素调控抗体分泌细胞游走到生殖道的分子机制和对局部抗体分泌的影响。方法:以鼠源杂交瘤细胞SG2和PA4为对象,检测其性激素受体、CXCR4的mRNA和CD31的表达;用流式细胞术和ELISA检测不同浓度性激素作用下,抗体分泌细胞表面与细胞游走相关分子的表达和抗体分泌的变化。结果:SG2和PA4细胞表达雄激素受体和雌激素受体β,表达CXCR4,但不表达CD31。性激素对抗体分泌细胞表面分子的表达和抗体分泌量并没有明显的影响。结论:性激素对抗体分泌细胞游走的调控作用与细胞自身相关黏附分子的变化关系不大,推测可能主要是通过对内皮细胞地址素表达的影响来实现的。Objective:To understand the mechanism that sex hormones regulate the migration of antibody-secreting cells to genital tracts and the effect of sex hormones on antibody production in mucosal associated tissues.Methods:We selected mouse SG2 and PA4 hybridoma cells respectively secreting IgG2b and polymer IgA,and used RT-PCR and flow cytometry for detecting expression of sex hormone receptors,CXCR4 mRNA and CD31 in SG2 and PA4 cells.The expression of surface molecular markers was measured by flow cytometry,and antibody production by SG2 or PA4 cells was determined using ELISA.Results:Androgen receptor,estrogen receptor β and CXCR4 mRNA were found in SG2 and PA4 cells,but CD31 were not expressed on these cells.The effects of sex hormones on the expression of surface molecular markers and antibody production by SG2 and PA4 cells were not obviously demonstrated.Conclusion:These results suggest that the adhesion molecules on the antibody-secreting cells are not associated with the regulation by sex hormone in the migration of these cells to genital tracts.Therefore,the migration regulation by sex hormones is most likely expected to be caused by the addressins on endothelial cells.

关 键 词：抗体分泌细胞 性激素受体 性激素 黏附分子 抗体 

分 类 号：R392.1[医药卫生—免疫学]