犬多价高免血清的研制及应用  

Preparation of canine multivalence serum and its application

作　　者：李志敏[1] 肖啸[1] 严达伟[1] 舒宏祥[1] 李建生[1] 

机构地区：[1]云南农业大学动物科学技术学院,昆明650201

出　　处：《中国免疫学杂志》2005年第7期521-523,共3页Chinese Journal of Immunology

摘　　要：目的:研制犬多价高免血清用于犬病毒性传染病的紧急预防和治疗。方法:选择1岁以上临床检查健康的昆明狼种犬及部分土犬20条,用犬瘟热病毒(CDV)、犬细小病毒(CPV)、犬传染性肝炎病毒(ICHV)、犬冠状病毒(CCV)、犬副流感病毒(CPIV)弱毒和强毒多次交叉免疫,颈动脉无菌采血,分离血清,实验室采用微量中和试验、血凝抑制试验来检测抗体效价。结果:应用本制品高免血清在临床上取得了较好的效果。结论:研制成稳定、高效的犬多价高免血清,并获得了较好的经济效益和社会效益。Objective:To prepare the canine multivalence serum for emergent prevation and theatmentor canines infection disease.Methods:Selecting 20 healthy Kunming dog,which is more than one years old.The canines were immuned by regulary with Canine distemper virus(CDV),Canine parvovirus virus(CPV),Infectious canine hepatitis virus(ICHV),Canine coronavirus(CCV),Canine parainflu-enza virus(CPIV).The blood were draw in freedom germ from neck's artery at canines and then blood was centrifugate for isolating serum.To check antibody's tite, micro serum neutrolization(SN) and hemagglutination inhibition(HI) were used.Results:Canine multivalence serum is successfully preparation and good curative effects in clinic.Conclusion:Canine multivalence serum may be of great value in economics and society and contribute to curative disease.

关 键 词：犬瘟热 犬细小病毒 犬传染性肝炎 犬副流感 犬冠状病毒肠炎 高免血清 

分 类 号：R392.9[医药卫生—免疫学]