系统性红斑狼疮患者树突状细胞亚型绝对数及与疾病活动程度相关性研究  

Study of the correlation between the absolute numbers of dendritic cells subsets and the disease activity in systemic lupus erythematosus patients

作　　者：齐晖 李富荣 刘冬舟 肖学吕 任莉莉 王新根 文锦丽 黄瑞芳 戴勇 

机构地区：[1]深圳市人民医院暨南大学第二附属医院临床医学研究中心,深圳518020 [2]深圳市人民医院暨南大学第二附属医院风湿免疫科,深圳518020

出　　处：《中国免疫学杂志》2005年第7期546-548,共3页Chinese Journal of Immunology

基　　金：广东省自然科学基金资助项目(021339)

摘　　要：目的:检测系统性红斑狼疮(SLE)患者外周血中树突状细胞亚型绝对数,分析其与疾病活动程度的相关性。方法:荧光染色标记DC,流式细胞仪检测分析DC亚型绝对数,并分析与SLEDAI、dsDNA、C3、C4的相关性。结果:SLE患者外周血MDC(13630.50±2548.55)和PDC(9196.29±1837.75)较正常对照组(15859.18±2550.55和11362.5±2100.14)明显降低(P<0.05)。静止期MDC和PDC(14503.06±2165.74和9564.75±1748.62)较对照组无明显下降,活动期组两DC亚型(12466.33±2600.32和8514.67±1918.73)较对照组下降明显(P<0.01)。SLE患者外周血MDC和PDC与SLEDAI、dsDNA明显负相关(P<0.01),与C3、C4呈正相关(P<0.05)。结论:DC参与疾病的发生,其绝对数可反映SLE疾病活动程度。Objective:To detect the absolute numbers of peripheral blood dendritic cells(DC) subsets in systemic lupus erythematosus(SLE) patients, and analyze the correlation between the disease activity.Methods:DC were labeled with fluorescence staining and the absolute numbers of DC subsets were measured by flow cytometer, and the correlations with SLEDAI, ds-DNA, C3 and C4 were analyzed.Results:The numbers of peripheral blood MDC(13 630.50±2 548.55) and PDC(9 196.29±1 837.75) in SLE patients decreased significantly than normal control groups(15 859.18±2 550.55, 11 362.5±2 100.14)(P<0.05). Numbers of MDC and PDC in quiescent period(14 503.06± 2 165.74, 9 564.75±1 748.62) had no significant difference with control groups, but numbers in active phase groups (12 466.33± 2 600.32,8 514.67±1 918.73) decreased significantly than control groups(P<0.01). The peripheral blood MDC and PDC in SLE patients were negatively correlated with SLEDAI and ds-DNA(P<0.01), but positively correlated with C3 and C4(P<0.05).Conclusion:DC are involved in the episode of disease, and its absolute numbers can reflect the degree of SLE activity.

关 键 词：树突状细胞亚型 红斑狼疮 系统性 疾病活动指数 

分 类 号：R593.2[医药卫生—内科学]