细胞因子诱导的杀伤细胞与肿瘤浸润淋巴细胞体外细胞毒活性比较  

Comparative study on cytotoxicity in vitro of cytokine-induced killer cells andtumor infiltrating lymphocytes

作　　者：蒋敬庭[1] 吴昌平[1] 邓海峰[1] 陆明洋[1] 李敏[1] 王赫[1] 吴骏[1] 

机构地区：[1]苏州大学附属第三医院肿瘤生物诊疗中心,江苏常州213003

出　　处：《检验医学》2005年第4期322-324,共3页Laboratory Medicine

基　　金：江苏省卫生厅重大科研课题基金资助项目(K200403);常州市科技局课题基金资助项目(CS2003207)

摘　　要：目的比较用多种细胞因子诱导的杀伤细胞(CIK)与癌性胸水中肿瘤浸润淋巴细胞(TIL)的细胞毒活性,为临床应用提供依据。方法用不同的细胞因子分别诱导培养CIK和癌性胸水TIL,按常规法计数细胞增殖量,采用四甲基偶氮唑盐(MTT)比色法检测其细胞毒活性。结果诱导培养14d后,CIK细胞毒活性为(76.65±16.78)%,增殖倍数为520±102;TIL细胞毒活性为(55.31±11.02)%,增殖倍数为182±78。结论CIK细胞对K562细胞的细胞毒活性在14d时达峰值,而TIL细胞在21d时才达峰值,且CIK细胞毒活性明显高于TIL细胞,增殖细胞数量也比TIL细胞高。CIK可替代TIL细胞进行胸腔注射治疗癌性胸水。Objective To compare the cytotoxicity of cytokine-induced killer cells(CIK) with the tumor infiltrating lymphocytes(TIL) on malignant pleural effusion, in order to clinical application. Methods To use the various cytokines to culture and guide the CIK and TILs of malignant pleural effusion, to count CIK cells and TILs proliferative index according to the traditional way, and to analyze the cytotoxicity by the MTT methods. Results After 14 days culture, the cytotoxicity and the increment of CIK were((76.65)±(16.78))% and 520±102, but the cytotoxicity and increment of TILs were((55.31)±(11.02))% and 182±(78). The peak value of the CIK cytotoxicity and TILs cytotoxicity to K_(562) were at 14th and day 21st day respecfively. Conclusions The cytotoxicity and the increment number of CIK are higher than those of TILs. The culture time of CIK in vitro is shorter. CIK is suitable to replace TILs by thorax injection and treat malignant pleural effusion.

关 键 词：细胞因子诱导的杀伤细胞 淋巴细胞 肿瘤浸润 细胞毒活性 

分 类 号：R446.62[医药卫生—诊断学]