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机构地区:[1]上海交通大学附属上海市第六人民医院检验科,上海200233
出 处:《检验医学》2005年第4期349-352,共4页Laboratory Medicine
基 金:上海市卫生局科技发展基金资助项目(01446)
摘 要:目的建立酶联免疫吸附试验(ELISA)检测尿半胱氨酸蛋白酶抑制剂C(CysC),探讨其在糖尿病肾病(DN)中的临床意义。方法检测129名正常对照、55例糖尿病(DM)患者、59例DN患者的血、尿CysC,59例DN组又分为A组[24h尿白蛋白(Alb)30~300mg/24h]39例,B组(Alb>300mg/24h)20例,比较各组结果,并绘制受试者工作特征(ROC)曲线分析其诊断效率。结果该方法的灵敏度为0.283μg/L。工作曲线的范围为0.39~25.0μg/L。A组与正常组比较,尿CysC差异有显著性(P<0.01),而血CysC差异无显著性(P>0.05);B组与A组比较,血、尿CysC差异均有显著性(P<0.01)。A组血、尿CysC的ROC曲线下面积分别为:0.448±0.081、0.811±0.055,B组血、尿CysC的ROC曲线下面积分别为0.813±0.060、0.928±0.037。结论本研究建立的ELISA方法检测尿CysC,灵敏度、稳定性及准确性高。研究结果表明,尿CysC对DN的诊断具有较高的敏感性及特异性。Objective To develop an enzym linked immuno sorbent assay (ELISA) for urinary cystatin C(Cys C) in order to evaluate its clinical significance in diabetic nephropathy (DN). Methods Assay the level of urinary and serum Cys C in 129 normal peoples, 55 patients with diabetic mellitus(DM) and 59 patients with DN. 59 DN patients were divided into two groups according to different urinary albumin(Alb) excretion, 30~300 mg/24 h(group A, 39 patients) and Alb>300 mg/24 h(group B, 20 patients). Results The detection limit of this assay was 0.283 μg/L. The working range was 0.39-25.0 μg/L. The urinary Cys C was significantly higher in A group than normal group (P<0.01). But the serum Cys C did not differ significantly between them(P>0.05). The level of serum and urinary Cys C were also obviously higher in B group than A group(P<0.01). The area of serum and urinary Cys C under the ROC curve of A group were 0.448±0.081 and 0.811±0.055, and the area of that under the ROC curve of B group were 0.813±0.060 and 0.928±0.037. Conclusions The ELISA for urinary Cys C had high sensitivity ,stability and accuracy. And it could be regard as a high sensitive and specific marker in DN.
关 键 词:尿半胱氨酸蛋白酶抑制剂C 糖尿病肾病 酶联免疫吸附试验(ELISA) 检测 糖尿病(DM) ELISA方法 ROC曲线 肾病(DN) CYS 显著性 临床意义 正常对照 尿白蛋白 诊断效率 曲线分析 工作特征 工作曲线 灵敏度 B组 受试者 正常组
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