Smad5基因在小鼠耳蜗中的表达及定位  

Expression and localization of Smad5 gene in mouse cochlea

作　　者：郭维[1] 杨仕明[1] 胡吟燕[1] 杨伟炎[1] 

机构地区：[1]解放军总医院耳鼻喉科,北京100853

出　　处：《解放军医学杂志》2005年第7期585-586,共2页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金(编号30000189);军队"十五"医药卫生科研基金(编号01Q050);北京市自然科学基金(编号7042061)资助课题

摘　　要：目的了解Smad5基因在小鼠耳蜗中的表达及定位。方法应用RTPCR检测Smad5基因的表达,用原位杂交法检测耳蜗中Smad5mRNA的表达部位,用免疫组织化学方法确定Smad5蛋白在耳蜗中的定位。结果Smad5基因在小鼠耳蜗中有高表达,表达部位在毛细胞、螺旋神经节细胞、支持细胞、血管纹、基底膜等。结论Smad5在耳蜗中存在,是耳蜗中众多蛋白中的一种。它可能在耳蜗的形成、毛细胞的分化过程中起着重要的作用。Objective To investigate the expression of Smad5 gene in the cochlea and to identify its location. Methods Reverse transcription polymerase chain reaction (RT-PCR) was employed to detect the expression of Smad5, the in situ hybridization and the immunohistochemical technique were used to localize the Smad5 messenger RNA and the Smad5 protein in mouse cochlea. Results Smad5 gene expressed in cochlea at a high level. Smad5 expression was concentrated in supporting cell, hair cell, spiral ganglion, epithelium of stria vascularis and basilar membrane. Conclusion The Smad5 proteins exist in the mouse cochlea and it may be involved in the cochlear formation and the differentiation of hair cell. Smad5 might be an essential factor for the development of normal cochlea.

关 键 词：基因 Smad5 耳蜗 原位杂交 

分 类 号：R764.3[医药卫生—耳鼻咽喉科]