不同生长因子对常温下体外灌流兔肾的影响  

Studies on the effects of different growth factors to perfused rabbit kidney in vitro

作　　者：谭红梅[1] 袁发焕[1] 

机构地区：[1]第三军医大学新桥医院肾内科,重庆400037

出　　处：《解放军医学杂志》2005年第7期619-622,共4页Medical Journal of Chinese People's Liberation Army

摘　　要：目的探讨不同生长因子对常温下体外灌流兔肾的影响。方法25只新西兰白兔随机分为5组,每组5只,分别用不同剂量的HGF、EGF及无生长因子的灌流培养液(对照组)于体外灌流兔肾,于灌流65h分别测量各组灌流兔肾静脉流出液LDH活性、Na+和K+浓度及尿NAG活性,以观测肾脏的功能。并行光镜下肾脏组织形态学和增殖细胞核抗原(PCNA)检查,观察肾组织形态改变和损伤程度。结果以高剂量HGF灌流的兔肾保存效果最好,其肾静脉流出液LDH活性、K+浓度、尿NAG活性,在灌流65h均显著低于其他组;肾静脉流出液Na+浓度显著高于其他各组。肾脏组织学、PCNA染色等检查,显示肾组织形态破坏及肾组织损伤较其他组轻。结论高剂量HGF用于常温下离体肾脏灌流,能显著改善保存效果,较好地保存灌流兔肾的功能和形态。Objective To investigate the effects of different growth factors to perfused kidney in vitro at room temperature. Methods 25 New Zealand rabbits were randomly divided into 5 groups, Each group had 5 rabbits. Perfusion was carried out in vitro on rabbits′ kidney with different culture media containing hepatocellular growth factor(HGF) in high or low dosage, epidermal growth factor(EGF) in high or low dosage, and growth factors-free. On the 65th hour after perfusion, lactate dehydrogenase(LDH) activity, the concentration of sodium and potassium in kidney vein effluent and β-N-acetyl amino glucosaminidase in urine (NAG) activity in each group were respectively detected to observe the renal function. Renal histological changes under light microscope and proliferating cell nuclear antigen (PCNA) of renal tissue were also examined to observe the renal morphological changes and the lesion degrees of renal tissue. Results The preservation effect of the culture media containing HGF in high dosage was the best among all 5 groups. In this group, LDH activity, the concentration of potassium in kidney vein effluent and NAG activity in urine were significantly lower than that of other 4 groups; the concentration of sodium of kidney vein effluent was notably higher than any other group. Renal histological changes under light microscope and PCNA stain of renal tissue showed that the renal morphological damage and the lesion of renal tissue were lighter than any other group. Conclusion HGF in high dosage could markedly improve the effect of perfusion preservation and protect the function and morphology structure of perfused rabbits′ kidneys better.

关 键 词：生长因子 肾功能衰竭 慢性 肾移植 灌流 保存 

分 类 号：R692.5[医药卫生—泌尿科学]