Molecular phylogenetics of Gymnocypris (Teleostei: Cyprinidae) in Lake Qinghai and adjacent drainages  

作　　者：ZHAOKai LIJunbing YANGGongshe DUANZiyuan HEShunping CHENYiyu 

机构地区：[1]InstituteofHydrobiology,ChineseAcademyofSciences,Wuhan430072,China//AgricultureandAnimalHusbandryCollege,QinghaiUniversity,Xining810003,China [2]InstituteofHydrobiology,ChineseAcademyofSciences,Wuhan430072,China [3]LaboratoryofAnimalFatDepositionandMuscleDevelopment,NorthwestSci-techUniversityofAgricultureandForestry,Yangling712100,China [4]InstituteofGeneticsandDevelopmentalBiology,ChineseAcademyofSciences,Beijing,100101,China

出　　处：《Chinese Science Bulletin》2005年第13期1325-1333,共9页

摘　　要：149 complete mitochondrial DNA (mtDNA) cytochrome b (Cyt b) genes (1140 bp) of Gymnocypris przewalskii, Gymnocypris eckloni and Gymnocypris scolisto-mus from the Lake Qinghai, Yellow River and Qaidam Basin were sequenced and analyzed. Consistent dendrogram indi-cated that the samples collected from the same species do not constitute a separate monophyletic group and all the samples were grouped into three highly divergent lineages (A, B and C). Among them, Lineage A contained all samples of G. przewalskii from the Lake Qinghai and partial samples of the G. eckloni from the Yellow River. Lineage B contained the remaining samples of G. eckloni from the Yellow River. Lineage C was composed of a monophyletic group by G. eck-loni from the Qaidam Basin. Analysis of molecular variance (AMOVA) indicated that most of genetic variations were detected within these three mtDNA lineages (93.12%), sug-gesting that there are three different lineages of Gymnocypris in this region. Our Cyt b sequence data showed that G. przewalskii was not a polytypic species, and G. scolistomus was neither an independent species nor a subspecies of G. eckloni. The divergent mtDNA lineages of G. eckloni from the Yellow River suggested that gene flow between the different populations was restricted to a certain extent by several gorges on the upper reach of the Yellow River. Lineage B of G. eckloni might be the genetic effect from the ancestor which was incorporated with the endemic schizothoracine fishes when the headward erosion of the Yellow River reached to its current headwaters of late. The G. eckloni from Basin Qaidam was a monophyletic group (lineage C) and Fst values within G. eckloni from the Yellow River were higher than 0.98, suggesting that the gene flow has been interrupted for a long time and the G. eckloni from Basin Qaidam might have been evolved into different species by ecology segrega-tion. The correlation between the rakers number of Gymno-cypris and population genetic variation was not significant. All Gymnocypris populations149 complete mitochondria! DMA (mtDNA) cytochrome b (Cyt b) genes (1140 bp)of Gymnocypris przewalskii, Gymnocypris eckloni and Gymnocypris scolistomus from the Lake Qinghai,Yellow Elver and Qaidam Basin were sequences! and analyzed. Consistent dendrogram indicated that thesamples collected from the same species do not constitute a separate mouopliyletk group and all thesamples were grouped into three highly divergent lineages (A, B and C). Among them, Lineage Acontained all samples of G. przewalskii from the Lake Qinghai and partial samples of the G. ecklonifrom the Yellow Eiver. Lineage B contained the remaining samples of G. eckloni from the YellowRiver. Lineage C was composed of a monophyletic group by G. eckloni from the Qaidam Basin. Analysisof molecular variance (AMOVA) indicated that most of genetic variations were detected within thesethree mtDNA lineages (93.12 percent), suggesting that there are three different lineages ofGymnocypris in this region. Oar Cyt b sequence data showed that G. przewalskii was not a polytypicspecies, and G. scolistomus was neither an independent species nor a subspecies of 6. eckloni. Thedivergent mtDNA lineages of G. eckloni from the Yellow River suggested that gene flow between thedifferent populations was restricted to a certain extent by several gorges on the upper reach of theYellow River. Lineage B of G. eckloni might be the genetic effect from the ancestor which wasincorporated with the endemic schizothoracine fishes when the headward erosion of the Yellow Riverreached to its current headwaters of late. The G. eckloni from Basin Qaidam was a monophyletic group(lineage C) and Fst values within G. eckloni from the Yellow River were higher than 0.98,suggesting that the gene flow has been Interrupted for a long time and the G. eckloni from BasinQaidam might have been evolved into different species by ecology segregation. The correlationbetween the takers number of Gymnocypris and population genetic variation was not significant. AllGymnocypris populations exhibited a low nu

关 键 词：分子学 系统发生学 细胞色素 DNA 

分 类 号：Q523[生物学—生物化学]