自制阳离子色谱柱测定有机胺  

Self-prepared cation-exchange column for the separation of amines

作　　者：陈永欣[1] 施青红 叶明立[1] 朱岩[1] 

机构地区：[1]浙江大学化学系,浙江杭州310028 [2]浙江工商大学食品生物与环境工程学院,浙江杭州310035

出　　处：《浙江大学学报（理学版）》2005年第4期415-418,共4页Journal of Zhejiang University（Science Edition）

基　　金：国家自然科学基金资助项目(20375025).

摘　　要：以乙基乙烯苯-二乙烯苯聚合物为基球,接枝羧酸基团合成制备了一种新型弱阳离子色谱柱填料.以硫酸作为淋洗液,用一价阳离子和低分子量有机胺对用该填料制成的色谱柱的色谱性能进行了测试,结果表明该柱填料对常见一价阳离子、铵和某些低分子量胺具有较好的分离效果、良好的重现性和低的检测限.A new chromatographic stationary phase of monodispersed pellicular cation exchanger was prepared. Monodispersed macroporous poly(ethlenevinylbenzene-co-divinylbenezene) beads of 6—8 μm diameter were prepared by a two-step swelling and polymerization method. Then carboxyl groups were introduced by polymerization of maleic anhydride with unreacted vinyl groups on the resin beads. The columns containing the new particles are 100% compatible with solvents commonly used for HPLC and can be used in a wide range of pH. Amines are used widely in chemical industry. The amounts of ammonium salts and trimethylamine are used to estimate the freshness of food. So it is important to detect amines in chemical and food industries. In this paper, the column packed with these carboxylate beads was used to separate monovalence metals, ammoniumion, and low-molecular weight amines in a single isocratic run by using sulfuric acid as eluent. The results showed the chromatographic stationary phase provided preferable resolution, low detection limit and good repeatability for these analyses. It's hoped that this new type of column can be used in practical sample detection.

关 键 词：阳离子色谱柱 填料 低分子量有机胺 

分 类 号：O657.7[理学—分析化学]