三种藻胆蛋白对人乳腺癌Bcap-37细胞的光动力杀伤效果  

Photodynamic effect of three kinds of phycobiliproteins on human mammary cancer line Bcap-37

作　　者：陈小强[1] 龚兴国[1] 梁倩[2] 史锋[1] 陈勇[1] 

机构地区：[1]浙江大学生命科学学院,浙江杭州310027 [2]浙江大学医学院,浙江杭州310016

出　　处：《浙江大学学报（理学版）》2005年第4期438-441,447,共5页Journal of Zhejiang University（Science Edition）

基　　金：国家技术创新基金资助项目(03C26213300586).

摘　　要：从高等红藻坛紫菜中提取R-藻红蛋白(R-PE)、R-藻蓝蛋白(R-PC),从低等蓝藻钝顶螺旋藻中提取C-藻蓝蛋白(C-PC).研究10、25、50和100μg/mL的R-PE、R-PC和C-PC介导的光动力效应对乳腺癌细胞Bcap-37的生存率的影响,MTT法测定结果,探讨这三种藻胆蛋白对癌细胞的光动力杀伤效应与其光敏性质差异的相关性.三种藻胆蛋白的光动力作用对Bcap-37细胞具有显著杀伤并显示出剂量效应,质量浓度在25μg/mL以上时,杀伤效果依次为:R-PE>R-PC>C-PC;仅用激光处理(497nm,632.8nm),Bcap-37细胞的生存率达到88.6%和91.3%;仅用藻胆蛋白处理,培养24h后,R-PE<25μg/mL,R-PC、C-PC<50μg/mL时,对Bcap-37细胞具有一定的促进生长作用,R-PE≥25μg/mL,R-PC、C-PC≥50μg/mL时,抑制细胞生长.R-Phycoerythrin(R-PE) and R-Phycocyanin(R-PC) were both obtained from red alga, Porphyra haitanensis,and C-Phycocyanin(C-PC) was obtained from blue-green alga Spirulina Platensis. In order to explore some relationship between the photodynamic effect on tumor cell and photodynamic action of these phycobiliproteins, different concentrations of these three phycobiliproteins(10,25,50 and 100 μg/mL)were used to study the photodynamic effect on human mammary cancer line Bcap-37, which was determined with MTT method. The Photodynamic effect on the cells was remarkable, demonstrating a dose-dependent response. Above the concentration of 25 μg/mL, the intensity of photodynamic effect is R-PE>R-PC>C-PC. The survival rates of the cells only treated by lasers(497 nm, 632.8 nm)were 88.6% and 91.3%. The growth of the cells only treated by phycobiliproteins and then cultured for 24 h was prompted with the lower concentration(R-PE<25 μg/mL,R-PC,C-PC<50 μg/mL) but inhibited with higher concentration(R-PE≥25 μg/mL,R-PC,C-PC≥50 μg/mL).

关 键 词：R-藻红蛋白 R-藻蓝蛋白 C-藻蓝蛋白 乳腺癌Bcap-37细胞 光动力学疗法 

分 类 号：Q631[生物学—生物物理学]