蓝细菌Anacystis nidulans R-2藻胆体中43kD蛋白细胞定位的初步研究  

Preliminary Studies on the 43-kD Protein in Phycobilisomes of Cyanobacterium Anacystis nidulans R-2

作　　者：李荣贵[1] 汪靖超[1] 

机构地区：[1]青岛大学生物系

出　　处：《植物学通报》2005年第3期302-306,共5页Chinese Bulletin of Botany

基　　金：国家自然科学基金项目(39525002;39570067)

摘　　要：高盐浓度条件下分离了蓝细菌AnacystisnidulansR-2的藻胆体,藻胆体中存在一种43kD的蛋白。Westernblotting分析表明,该蛋白能与蓝细菌Fd:NADP+氧还酶中FNR结构域的抗体发生反应,解聚的藻胆体具有FNR黄递酶的活性,初步证明该43kD蛋白就是Fd:NADP+氧还酶。TritonX-114分相实验表明,这种43kD的蛋白不能进入TritonX-114相。对藻胆体的部分解聚合实验表明,富含外周杆的组分中不存在43kD的蛋白。Phycobilisomes, containing a 43-kD protein, were isolated from Anacystis nidulans R-2. Dissociated phycobilisomes exhibited FNR diaphorase activity. Western blotting showed that the 43-kD protein could react with antibodies raised against Fd:NADP+ oxidoreductase. Analysis by Triton X-114 phase-partitioning indicated that the 43-kD protein could not enter into the detergent phase. Partial dissociation of phycobilisomes revealed no 43-kD protein in the fractions mainly containing rod components.

关 键 词：43kD蛋白 藻胆体 蓝细菌 细胞定位 TRITON Western 度条件 分析表 黄递酶 FNR Fd 实验 

分 类 号：Q936.1[生物学—微生物学]