小麦Rubisco活化酶基因的克隆和表达特性  

Cloning and Expression of Rubisco Activase Gene in Wheat

作　　者：张国[1] 李滨[1] 邹琦[1] 

机构地区：[1]山东农业大学生命科学学院植物系,泰安271018

出　　处：《植物学通报》2005年第3期313-319,共7页Chinese Bulletin of Botany

基　　金：国家重点基础研究发展规划项目(G1998010100)

摘　　要：Rubisco活化酶是广泛存在于光合生物中调节Rubisco活性的酶,我们利用PCR技术,从小麦(Triticumaestivum)叶片cDNA文库中克隆得到Rubisco活化酶基因cDNA片段,该片段长度为850bp,编码201个氨基酸。Northernblot表明,小麦叶片在暗诱导衰老的条件下,叶片中活化酶基因表达水平逐渐下降;同时,小麦叶片的光合特性、叶绿素含量和Rubisco活性呈现下降趋势。这些结果表明,衰老时小麦叶片Rubisco活化酶基因表达水平下降与光合速率下降密切相关。Rubisco activase is an ubiquitous enzyme for the activation of Rubisco in photosynthetic autotrophs. A cDNA fragment of Rubisco activase gene was cloned from wheat (Triticum aestivum). Northern blot showed that expression of the gene was down-regu- lated in dark-induced senescence leaves, where photosynthetic rate, chlorophyll content and Rubisco activity also showed obvious decline. The results suggest that the decreased expres- sion level of the gene was related to the decline in photosynthetic rate.

关 键 词：酶基因 RUBISCO活化酶 表达特性 Rubisco活性 克隆 Northern 基因表达水平 CDNA片段 CDNA文库 小麦叶片 PcR技术 叶绿素含量 光合生物 片段长度 BLOT 光合特性 光合速率 下降 氨基酸 衰老 

分 类 号：S512.1[农业科学—作物学]